Document Detail


High level expression of active human alpha-1-antitrypsin in the milk of transgenic sheep.
MedLine Citation:
PMID:  1367357     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe the generation of five sheep transgenic for a fusion of the ovine beta-lactoglobulin gene promotor to the human alpha 1-antitrypsin (h alpha 1AT) genomic sequences. Four of these animals are female and one male. Analysis of the expression of h alpha 1AT in the milk of three of these females shows that all express the human protein at levels greater than 1 gram per liter. In one case initial levels exceeded 60 grams per liter and stabilized at approximately 35 grams per liter as lactation progressed. Human alpha 1AT purified from the milk of these animals appears to be fully N-glycosylated and has a biological activity indistinguishable from human plasma-derived material.
Authors:
G Wright; A Carver; D Cottom; D Reeves; A Scott; P Simons; I Wilmut; I Garner; A Colman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bio/technology (Nature Publishing Company)     Volume:  9     ISSN:  0733-222X     ISO Abbreviation:  Biotechnology (N.Y.)     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1991-10-10     Completed Date:  1991-10-10     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8309273     Medline TA:  Biotechnology (N Y)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  830-4     Citation Subset:  B    
Affiliation:
Pharmaceutical Proteins Limited, Edinburgh, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Blotting, Southern
DNA / genetics,  isolation & purification
Female
Glycosylation
Humans
Lactation
Lactoglobulins / genetics
Male
Milk / chemistry*
Molecular Weight
Promoter Regions, Genetic
Sheep
Trypsin / metabolism
alpha 1-Antitrypsin / analysis,  genetics*,  pharmacology
Chemical
Reg. No./Substance:
0/Lactoglobulins; 0/alpha 1-Antitrypsin; 9007-49-2/DNA; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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