| High-level expression of Rhodotorula gracilis D: -amino acid oxidase in Pichia pastoris. | |
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MedLine Citation:
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PMID: 21053050 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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By combining gene design and heterologous over-expression of Rhodotorula gracilis D: -amino acid oxidase (RgDAO) in Pichia pastoris, enzyme production was enhanced by one order of magnitude compared to literature benchmarks, giving 350 kUnits/l of fed-batch bioreactor culture with a productivity of 3.1 kUnits/l h. P. pastoris cells permeabilized by freeze-drying and incubation in 2-propanol (10% v/v) produce a highly active (1.6 kUnits/g dry matter) and stable oxidase preparation. Critical bottlenecks in the development of an RgDAO catalyst for industrial applications have been eliminated. |
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Authors:
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Sandra Abad; Jozef Nahalka; Margit Winkler; Gabriele Bergler; Robert Speight; Anton Glieder; Bernd Nidetzky |
Publication Detail:
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Type: Journal Article Date: 2010-11-04 |
Journal Detail:
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Title: Biotechnology letters Volume: 33 ISSN: 1573-6776 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-02-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 557-63 Citation Subset: IM |
Affiliation:
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Austrian Centre of Industrial Biotechnology, Petersgasse 14, Graz, 8010, Austria. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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