Document Detail

High-level expression of the 32.5-kilodalton calelectrin in ductal epithelia as revealed by immunocytochemistry.
MedLine Citation:
PMID:  2952539     Owner:  NLM     Status:  MEDLINE    
Calelectrins are a family of antigenically related Ca2+-binding proteins that have only recently been described. They have the important property of binding to membranes only in the presence of Ca2+. We systematically studied the tissue localization of one calelectrin, the 32.5-kilodalton species, in rats using immunocytochemistry. We found that high levels were exclusively present in the epithelial cells of bile and pancreatic ducts, renal collecting ducts, bronchial epithelia, and brain ependyma. In all of these organs, the other cells were not immunoreactive. In addition, strong immunoreactivity was found in the intercalated disks of myocardial cells, and mild immunoreactivity was observed in several endocrine tissues. In contrast, the cellular distribution of the 67-kilodalton calelectrin was more diffuse, involving most parenchymal cells in addition to the already-mentioned cells. Due to the presence of high levels of 32.5-kilodalton calelectrin in some cell types, this protein may be used as a histochemical marker for differentiated ductal epithelial cells, some specialized epithelia, myocardial cells, and Paneth cells.
F G Silva; K Sherrill; S Spurgeon; T C Südhof; D K Stone
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  33     ISSN:  0301-4681     ISO Abbreviation:  Differentiation     Publication Date:  1986  
Date Detail:
Created Date:  1987-05-27     Completed Date:  1987-05-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  175-83     Citation Subset:  IM    
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MeSH Terms
Calcium-Binding Proteins / analysis*,  biosynthesis
Epithelial Cells
Epithelium / metabolism
Immune Sera
Immunoenzyme Techniques
Molecular Weight
Nerve Tissue Proteins / analysis*
Tissue Distribution
Grant Support
Reg. No./Substance:
0/Annexins; 0/Calcium-Binding Proteins; 0/Immune Sera; 0/Nerve Tissue Proteins

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