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High level of dietary zinc oxide change the bacterial core and enterobacterial composition in the ileum of piglets.
MedLine Citation:
PMID:  21383037     Owner:  NLM     Status:  Publisher    
This study was conducted to investigate the effects of high level of dietary ZnO on the bacterial core and enterobacterial composition in the small intestine of piglets, and they were fed diets containing a total of 124 or 3,042 mg Zn per kg diet, respectively. Zinc was supplemented to the basal diet as ZnO. Bacterial 16S rRNA genes of ileal DNA extracts were PCR amplified with 2 bar coded primer sets and sequenced by 454 pyrosequencing. The bacterial core species were calculated from the relative abundances of reads present in 5 of 6 samples per group and at a minimum of 5 sequences per sample. The reference database SILVA was used to assign sequence reads at an alignment minimum of 200 bases and 100% identity. Lactic acid bacteria dominated the bacterial core, but showed diverse responses to dietary ZnO. Of the dominant Lactobacillus spp., Lactobacillus reuteri was reduced due to high dietary ZnO (44.7 vs. 17.9%; P = 0.042), but L. amylovorus was not influenced. However, the changes of relative abundances of other lactic acid bacteria were more noteworthy , as Weissella cibaria (10.7 vs. 23.0%; P = 0.006), Weissella confusa (10.0 vs. 22.4%; P = 0.037), Leuconostoc citreum (6.5 vs. 14.8%; P = 0.009), Streptococcus equinus (0.14 vs. 1.0%; P = 0.044), and S. lutetiensis (0.01 vs. 0.11%; P = 0.016) increased in relative abundance. Non lactic acid bacteria that were influenced by high dietary ZnO included the strict anaerobic species, Sarcina ventriculi, which showed a strong numerical decrease in relative abundance (14.6 vs. 5.1%). Species of the Enterobacteriaceae increased their relative abundance, as well as species diversity, in the high dietary ZnO experimental group. Bacterial diversity indices were increased due to high dietary ZnO (P < 0.05), which was traced back to the increase of sequences from subdominant species. High dietary ZnO led to an increase of less prominent species and, thus, had a major impact on the bacterial composition and diversity in piglets. This effect may help to stabilize the intestinal microbiota in the sensitive post-weaning period.
W Vahjen; R Pieper; J Zentek
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-7
Journal Detail:
Title:  Journal of animal science     Volume:  -     ISSN:  1525-3163     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Institute of Animal Nutrition, Department of Veterinary Medicine, Freie Universität Berlin, 14195 Berlin, Germany.
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