| High-level cryIVD and cytA gene expression in Bacillus thuringiensis does not require the 20-kilodalton protein, and the coexpressed gene products are synergistic in their toxicity to mosquitoes. | |
| | |
MedLine Citation:
|
PMID: 8481007 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Interactions among the 20-kDa protein gene and the cytA and cryIVD genes located in a 9.4-kb HindIII fragment were studied. A series of plasmids containing a combination of these different genes was constructed by using the Escherichia coli/Bacillus thuringiensis shuttle vector pHT3101. The plasmids were then used to transform an acrystalliferous strain, cryB, derived from B. thuringiensis subsp. kurstaki. The results from sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analyses suggest that although the 20-kDa protein is required for the efficient CytA protein production in E. coli, it is not required in B. thuringiensis. With or without the truncated 20-kDa protein gene, the CtyA and/or CryIVD proteins are produced and form parasporal inclusions in B. thuringiensis cells. However, more-efficient expression is obtained when a second protein, probably acting as a chaperonin, is present. In addition, the time course studies show that the CytA and CryIVD proteins are coordinately produced. Both the crude B. thuringiensis culture and purified inclusions from each recombinant B. thuringiensis strain are toxic to Culex quinquefasciatus larvae. The parasporal inclusions formed in B. thuringiensis cells are mosquitocidal, with CytA synergizing CryIVD toxicity. |
| | |
Authors:
|
C Chang; Y M Yu; S M Dai; S K Law; S S Gill |
Related Documents
:
|
11010897 - Molecular genetic manipulation of truncated cry1c protein synthesis in bacillus thuring... 12198307 - Preliminary x-ray crystallographic analysis of a soluble form of mntc, a periplasmic ma... 19780817 - Essential role of the c-terminus in melanocarpus albomyces laccase for enzyme productio... 10771437 - Expression, purification, crystallization and preliminary x-ray diffraction studies of ... 21221937 - Rescue of a cold-sensitive mutant at low temperatures by cold shock proteins from polar... 8486617 - The coated vesicle vacuolar (h+)-atpase associates with and is phosphorylated by the 50... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Applied and environmental microbiology Volume: 59 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 1993 Mar |
Date Detail:
|
Created Date: 1993-05-27 Completed Date: 1993-05-27 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 815-21 Citation Subset: IM |
Affiliation:
|
Department of Entomology, University of California, Riverside 92521. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Bacillus thuringiensis / genetics* Bacterial Proteins / biosynthesis, genetics*, metabolism* Bacterial Toxins / biosynthesis, genetics* Culex Deoxyribonuclease HindIII Drug Synergism Electrophoresis, Polyacrylamide Gel Endotoxins* Gene Expression Regulation, Bacterial Genes, Bacterial Hemolysin Proteins Immunoblotting Pest Control, Biological Plasmids |
| Grant Support | |
ID/Acronym/Agency:
|
ESO3298/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Proteins; 0/Bacterial Toxins; 0/Endotoxins; 0/Hemolysin Proteins; 0/insecticidal crystal protein, Bacillus Thuringiensis; EC 3.1.21.-/Deoxyribonuclease HindIII |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Identification of metabolites from the degradation of fluoranthene by Mycobacterium sp. strain PYR-1...
Next Document: Aspartate aminotransferase and tylosin biosynthesis in Streptomyces fradiae.