Document Detail


High hydrostatic pressure induces counterclockwise to clockwise reversals of the Escherichia coli flagellar motor.
MedLine Citation:
PMID:  23417485     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The bacterial flagellar motor is a reversible rotary machine that rotates a left-handed helical filament, allowing bacteria to swim toward a more favorable environment. The direction of rotation reverses from counterclockwise (CCW) to clockwise (CW), and vice versa, in response to input from the chemotaxis signaling circuit. CW rotation is normally caused by binding of the phosphorylated response regulator CheY (CheY-P), and strains lacking CheY are typically locked in CCW rotation. The detailed mechanism of switching remains unresolved because it is technically difficult to regulate the level of CheY-P within the concentration range that produces flagellar reversals. Here, we demonstrate that high hydrostatic pressure can induce CW rotation even in the absence of CheY-P. The rotation of single flagellar motors in Escherichia coli cells with the cheY gene deleted was monitored at various pressures and temperatures. Application of >120 MPa pressure induced a reversal from CCW to CW at 20°C, although at that temperature, no motor rotated CW at ambient pressure (0.1 MPa). At lower temperatures, pressure-induced changes in direction were observed at pressures of <120 MPa. CW rotation increased with pressure in a sigmoidal fashion, as it does in response to increasing concentrations of CheY-P. Application of pressure generally promotes the formation of clusters of ordered water molecules on the surfaces of proteins. It is possible that hydration of the switch complex at high pressure induces structural changes similar to those caused by the binding of CheY-P.
Authors:
Masayoshi Nishiyama; Yoshiyuki Sowa; Yoshifumi Kimura; Michio Homma; Akihiko Ishijima; Masahide Terazima
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-02-15
Journal Detail:
Title:  Journal of bacteriology     Volume:  195     ISSN:  1098-5530     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-29     Completed Date:  2013-05-23     Revised Date:  2013-10-08    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1809-14     Citation Subset:  IM    
Affiliation:
The Hakubi Center for Advanced Research, Kyoto University, Kyoto, Japan. mnishiyama@icems.kyoto-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics,  metabolism
Biomechanics
Escherichia coli / genetics,  physiology*
Flagella / physiology*
Gene Expression Regulation, Bacterial / physiology
Hydrostatic Pressure
Membrane Proteins / genetics,  metabolism
Molecular Motor Proteins / physiology*
Movement / physiology
Protein Binding
Rotation
Temperature
Thermodynamics
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Membrane Proteins; 0/Molecular Motor Proteins; 0/methyl-accepting chemotaxis proteins
Comments/Corrections

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