Document Detail


High hydrostatic pressure and the dissection of fertilization responses. I. The relationship between cortical granule exocytosis and proton efflux during fertilization of the sea urchin egg.
MedLine Citation:
PMID:  6307729     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
High hydrostatic pressure applied between sperm attachment and the onset of cortical granule exocytosis will inhibit this exocytotic event in sea urchin eggs. Such pressure-treated zygotes, nevertheless, are activated and capable of development. Thus, this technique can be used as a tool to study the relationship between cortical granule breakdown and other fertilization-related responses. We have studied whether the exocytosis of cortical granules is necessary for proton efflux (acid release) to occur. Our results indicate that although Ca2+ is released while the eggs are under pressure (a prerequisite for the following events to take place), cortical granule exocytosis and acid release are pressure-sensitive and completely inhibited at pressures above 400 atm (6000 psi) and 275 atm (4000 psi), respectively. However, upon decompression, acid release is initiated which amounts to 65-70% of that seen in the unpressurized controls, suggesting that the efflux mechanism does not require cortical granule exocytosis and must result from some modification of the original plasma membrane of the egg. The remaining 30-35% of the acid release is related to cortical granule exocytosis, since it can be obtained upon induction of the cortical granule fusion 30 min later under atmospheric pressure. The initiation of acid release after decompression indicates that the efflux mechanism is not transiently turned on at fertilization, but undergoing long-term modification; the recovery of the ability to induce cortical granule fusion after fertilization under pressure suggests a refilling of cytoplasmic Ca2+ stores within this time course.
Authors:
T Schmidt; D Epel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  146     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1983 Jul 
Date Detail:
Created Date:  1983-09-09     Completed Date:  1983-09-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  235-48     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcimycin / pharmacology
Calcium / metabolism
Exocytosis
Female
Fertilization*
Hydrogen / metabolism*
Hydrogen-Ion Concentration
Hydrostatic Pressure*
Kinetics
Male
Ovum / physiology*
Pressure*
Protons
Sea Urchins
Spermatozoa / physiology
Chemical
Reg. No./Substance:
0/Protons; 1333-74-0/Hydrogen; 52665-69-7/Calcimycin; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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