| High hydrostatic pressure approach proves RNA catalytic activity without magnesium. | |
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MedLine Citation:
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PMID: 17222901 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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High hydrostatic pressure (HHP) technique was used to evaluate a mechanism of RNA hydrolysis with RNA. We showed that hammerhead ribozyme specifically cleaves RNA substrate at HHP in the absence of Mg(2+). A deoxyribozyme "10-23" was active in the same conditions. These results pointed out that the hydrolytic activity of nucleic acid depends on proper tertiary structure of a complex with a substrate. They prove that magnesium ion is not directly involved in catalysis process. On that basis we show the mechanism of RNA hydrolysis catalyzed with nucleic acids at HHP. |
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Authors:
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Agnieszka Fedoruk-Wyszomirska; Eliza Wyszko; Małgorzata Giel-Pietraszuk; Mirosława Z Barciszewska; Jan Barciszewski |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-12-17 |
Journal Detail:
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Title: International journal of biological macromolecules Volume: 41 ISSN: 0141-8130 ISO Abbreviation: Int. J. Biol. Macromol. Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-03-30 Completed Date: 2007-05-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7909578 Medline TA: Int J Biol Macromol Country: England |
Other Details:
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Languages: eng Pagination: 30-5 Citation Subset: IM |
Affiliation:
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Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Hydrolysis Hydrostatic Pressure Magnesium / metabolism Molecular Sequence Data Nucleic Acid Conformation RNA, Catalytic / chemistry*, genetics, metabolism* RNA, Viral / chemistry, genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/RNA, Catalytic; 0/RNA, Viral; 7439-95-4/Magnesium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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