| High hydrogen peroxide concentration in the feed-zone affects bioreactor cell productivity with liquid phase oxygen supply strategy. | |
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MedLine Citation:
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PMID: 17972108 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Liquid phase oxygen supply strategy (LPOS), in which hydrogen peroxide (H(2)O(2)) is used to supply oxygen to the bioreactor, leads to low cell productivity despite high specific productivities of relevant metabolites. We hypothesized that high H(2)O(2) concentrations in the feed-zone led to local cell death, which in turn, lead to lower cell productivity. To test the hypothesis, a mathematical model was developed. Bacillus subtilis 168 was used as the model system in this study. The model simulations of cell concentrations in the bioreactor-zone were verified with the experimental results. The feed-zone H(2)O(2) concentrations remained 12-14 times higher than bulk bioreactor concentrations. The high local concentrations are expected to cause local cell killing, which explains the decrease in overall cell production by 50% at 300 rpm compared to conventional cultivation. Further, among the four different feed strategies studied using the model, dissolved oxygen (DO) controlled H(2)O(2) feed strategy caused least local cell killing and improved overall cell production by 34%. |
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Authors:
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Pritish Sarkar; Kaushik Ghosh; G K Suraishkumar |
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Publication Detail:
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Type: Journal Article Date: 2007-10-31 |
Journal Detail:
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Title: Bioprocess and biosystems engineering Volume: 31 ISSN: 1615-7591 ISO Abbreviation: Bioprocess Biosyst Eng Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-05-16 Completed Date: 2008-08-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101088505 Medline TA: Bioprocess Biosyst Eng Country: Germany |
Other Details:
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Languages: eng Pagination: 357-67 Citation Subset: IM |
Affiliation:
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Department of Biotechnology, Indian Institute of Technology Madras, Bhupat and Jyoti Mehta School of Biosciences Building, Chennai, 600 036, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus subtilis
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growth & development*,
metabolism* Bioreactors / microbiology* Cell Culture Techniques / methods* Hydrogen Peroxide / metabolism* Models, Biological* Oxygen / metabolism* Phase Transition Solutions |
| Chemical | |
Reg. No./Substance:
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0/Solutions; 7722-84-1/Hydrogen Peroxide; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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