Document Detail

High-grade Glioma Motility Reduced by Genetic Knockdown of KCC3.
MedLine Citation:
PMID:  22776998     Owner:  NLM     Status:  Publisher    
Cell motility is dependent on a coordinated reorganization of the cytoskeleton, membrane recycling, and focal adhesion to the extracellular matrix. Each of these cellular processes involves re-distribution of cell water, which is facilitated by the transport of inorganic ions (with obligatory water movement). Scratch-wound healing assays of Wistar C6 glioblastoma cells demonstrated cell motility in advance of cell proliferation. Although bumetanide inhibition of Na-K-2Cl cotransport activity did not affect cell motility, treatment of glioma cells with furosemide to inhibit K-Cl cotransport activity prevented ∼75% of wound closure in a reversible reaction. Genetic silencing of KCC3 with short hairpin interfering RNA reduced protein expression by 40 - 60%, K(+) influx by ∼50%, and cell motility by ∼50%. Appearance of KCC1 mRNA and KCC3 mRNA at 25 PCR cycles versus KCC4 mRNA at 35 PCR cycles, suggests more KCC1/KCC3 expression in both primary rat astrocytes and C6 glioma cells. Altogether, these experiments suggest that the presence/function of multiple isoforms of the Na(+-)independent K-Cl cotransporter may have a role in glioma cell motility.
Kenneth B Gagnon
Related Documents :
7165278 - A simple photometric microassay for the quantitative evaluation of macrophage-mediated ...
6716398 - Synthesis of pyridine derivatives of l-phenylalanine as antisickling reagents.
20177898 - Erucylphospho-n,n,n-trimethylpropylammonium (erufosine) is a potential antimyeloma drug...
25479178 - Intraepithelial t-cell cytotoxicity, induced bronchus-associated lymphoid tissue, and p...
12457038 - Proestrous surge of gonadotropin-releasing hormone secretion inhibits apoptosis of ante...
24962088 - Assessing species-specific contributions to craniofacial development using quail-duck c...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-06
Journal Detail:
Title:  Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology     Volume:  30     ISSN:  1421-9778     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9113221     Medline TA:  Cell Physiol Biochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  466-476     Citation Subset:  -    
Copyright Information:
Copyright © 2012 S. Karger AG, Basel.
Department of Anesthesiology, Vanderbilt University School of Medicine, Nashville, TN, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Thermally induced [3+2] cyclization of aniline-tethered alkylidenecyclopropanes: a facile synthetic ...
Next Document:  Perioperative Factors Associated with Prolonged Intensive Care Unit and Hospital Length of Stay afte...