Document Detail


High-grade Glioma Motility Reduced by Genetic Knockdown of KCC3.
MedLine Citation:
PMID:  22776998     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Cell motility is dependent on a coordinated reorganization of the cytoskeleton, membrane recycling, and focal adhesion to the extracellular matrix. Each of these cellular processes involves re-distribution of cell water, which is facilitated by the transport of inorganic ions (with obligatory water movement). Scratch-wound healing assays of Wistar C6 glioblastoma cells demonstrated cell motility in advance of cell proliferation. Although bumetanide inhibition of Na-K-2Cl cotransport activity did not affect cell motility, treatment of glioma cells with furosemide to inhibit K-Cl cotransport activity prevented ∼75% of wound closure in a reversible reaction. Genetic silencing of KCC3 with short hairpin interfering RNA reduced protein expression by 40 - 60%, K(+) influx by ∼50%, and cell motility by ∼50%. Appearance of KCC1 mRNA and KCC3 mRNA at 25 PCR cycles versus KCC4 mRNA at 35 PCR cycles, suggests more KCC1/KCC3 expression in both primary rat astrocytes and C6 glioma cells. Altogether, these experiments suggest that the presence/function of multiple isoforms of the Na(+-)independent K-Cl cotransporter may have a role in glioma cell motility.
Authors:
Kenneth B Gagnon
Related Documents :
16848238 - Apoptotic death of human lympholeukemia hl-60 cells resultant from combined effect of c...
19538468 - Anti-proliferative effect of rhein, an anthraquinone isolated from cassia species, on c...
20038138 - Mechanistic insight into cell growth, internalization, and cytotoxicity of pamam dendri...
3536928 - A serine protease triggers the initial step of transmembrane signalling in cytotoxic t ...
70948 - The nature of dendritic cells of the epidermis of the white guinea pig.
7885078 - Genetically engineered in vitro systems for biotransformation studies.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-06
Journal Detail:
Title:  Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology     Volume:  30     ISSN:  1421-9778     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9113221     Medline TA:  Cell Physiol Biochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  466-476     Citation Subset:  -    
Copyright Information:
Copyright © 2012 S. Karger AG, Basel.
Affiliation:
Department of Anesthesiology, Vanderbilt University School of Medicine, Nashville, TN, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Thermally induced [3+2] cyclization of aniline-tethered alkylidenecyclopropanes: a facile synthetic ...
Next Document:  Perioperative Factors Associated with Prolonged Intensive Care Unit and Hospital Length of Stay afte...