Document Detail


High-fructose diet elevates myocardial superoxide generation in mice in the absence of cardiac hypertrophy.
MedLine Citation:
PMID:  19932004     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Dietary fructose intake has increased considerably in recent decades and this has been paralleled by an increase in the incidence of insulin resistance, especially in children and adolescents. The impact of a high-fructose diet on the myocardium is not fully understood. The aims of this study were to characterize the murine metabolic and cardiac phenotypes associated with a high-fructose diet and to determine whether this diet imparts differential effects with age.
METHODS: Juvenile (4 wk) and adult (14 wk) C57Bl/6 mice were fed a 60% fructose diet or isoenergetic control (starch) diet for 6 wk.
RESULTS: At completion of the dietary intervention (at ages 10 and 20 wk), fructose-fed mice were normotensive; hyperinsulinemia and cardiac hypertrophy were not evident. Interestingly, fructose-fed mice exhibited lower blood glucose levels (10 wk: 4.81+/-0.28 versus 5.42+/-0.31 mmol/L; 20 wk: 4.88+/-0.30 versus 5.96+/-0.42 mmol/L, P<0.05) compared with controls. Nicotinamide adenosine dinucleotide phosphate-driven myocardial superoxide production was significantly increased in fructose-fed mice at both ages (by approximately 29% of control at 10 wk of age and 16% at 20 wk, P<0.01). No increase in aortic superoxide production was observed. Fructose feeding did not alter gene expression of the antioxidant thioredoxin-2, suggesting an imbalance between myocardial reactive oxygen species generation and antioxidant induction.
CONCLUSION: These findings indicate that increased myocardial superoxide production may represent an early and primary cardiac pathologic response to the metabolic challenge of excess dietary fructose in juveniles and adults that can be detected in the absence of cardiac hypertrophy and hypertension.
Authors:
Kimberley Mellor; Rebecca H Ritchie; Greta Meredith; Owen L Woodman; Margaret J Morris; Lea M D Delbridge
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-20
Journal Detail:
Title:  Nutrition (Burbank, Los Angeles County, Calif.)     Volume:  26     ISSN:  1873-1244     ISO Abbreviation:  Nutrition     Publication Date:    2010 Jul-Aug
Date Detail:
Created Date:  2010-06-28     Completed Date:  2011-01-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8802712     Medline TA:  Nutrition     Country:  United States    
Other Details:
Languages:  eng     Pagination:  842-8     Citation Subset:  IM    
Copyright Information:
2010 Elsevier Inc. All rights reserved.
Affiliation:
Department of Physiology, University of Melbourne, Melbourne, Victoria, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta / metabolism
Blood Glucose / metabolism*
Blood Pressure / drug effects
Cardiomegaly
Dietary Sucrose / administration & dosage*
Fructose / pharmacology*
Gene Expression / drug effects
Heart / drug effects*
Insulin / blood*
Male
Mice
Mice, Inbred C57BL
Myocardium / metabolism*,  pathology
NADP / metabolism
Superoxides / metabolism*
Thioredoxins / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Dietary Sucrose; 11061-68-0/Insulin; 11062-77-4/Superoxides; 30237-26-4/Fructose; 52500-60-4/Thioredoxins; 53-59-8/NADP

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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