Document Detail


High frequency of DD polymorphism of the angiotensin-converting enzyme gene in Turkish asthmatic patients.
MedLine Citation:
PMID:  15510584     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study is to detect the incidence of the angiotensin-converting enzyme (ACE) gene polymorphism in Turkish asthmatic patients and to examine whether there is an association between the disease and ACE gene polymorphism. In our study, the genomic DNA of 100 asthmatic patients and 88 healthy subjects was analyzed Genomic DNA was isolated from peripheral blood by using standard methods. The intron 16 of the ACE gene was amplified by the polymerase chain reaction (PCR) method using primers ACE and ACEX to examine the presence and absence of a 287-base pair (bp) DNA fragment that showed I/D polymorphism genotypes. PCR products were separated by agarose gel electrophoresis and were visualized by a charge-coupled device camera. Serum ACE activities were measured using an ACE kit. The results were evaluated statistically using the chi-square test and one-way analysis of variance. Although the population of patients with asthma was characterized by a higher frequency (30%) of the DD genotype of ACE, they were characterized by lower frequency (48%) of the ID genotype of ACE (DD, 16%, and ID, 64%, in healthy control subjects). The frequency of the I and D alleles of the ACE gene was not significantly different between asthmatic patients (0.46/0.54) and healthy controls (0.52/ 0.48). In addition, in both asthmatic patients and controls, there was a significant decrease of the levels of ACE activity in individuals that have II genotypes when compared with individuals that have DD genotypes. ACE activities were increased significantly in all asthmatic patients (67.20 +/- 1.95 IU/L) compared with all healthy controls (60.90 +/- 2.12 IU/L).
Authors:
Meral Urhan; Irfan Degirmenci; Emel Harmanci; Hasan V Gunes; Muzaffer Metintas; Ayse Basaran
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Allergy and asthma proceedings : the official journal of regional and state allergy societies     Volume:  25     ISSN:  1088-5412     ISO Abbreviation:  Allergy Asthma Proc     Publication Date:    2004 Jul-Aug
Date Detail:
Created Date:  2004-10-29     Completed Date:  2005-02-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9603640     Medline TA:  Allergy Asthma Proc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  243-7     Citation Subset:  IM    
Affiliation:
Institute of Health Sciences, Department of Medical Biology, Osmangazi University, Eskisehir, Turkey.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Asthma / enzymology,  genetics*
Base Pairing / genetics
Case-Control Studies
Female
Genotype
Humans
Introns / genetics
Male
Middle Aged
Peptidyl-Dipeptidase A / genetics*,  metabolism
Polymorphism, Genetic / genetics*
Turkey
Chemical
Reg. No./Substance:
EC 3.4.15.1/Peptidyl-Dipeptidase A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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