| High field magnetic resonance microscopy of the human hippocampus in Alzheimer's disease: Quantitative imaging and correlation with iron. | |
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MedLine Citation:
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PMID: 21867761 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We report R(2) and R(2)* in human hippocampus from five unfixed post-mortem Alzheimer's disease (AD) and three age-matched control cases. Formalin-fixed tissues from opposing hemispheres in a matched AD and control were included for comparison. Imaging was performed in a 600MHz (14T) vertical bore magnet at MR microscopy resolution to obtain R(2) and R(2)* (62μm×62μm in-plane, 80μm slice thickness), and R(1) at 250μm isotropic resolution. R(1), R(2) and R(2)* maps were computed for individual slices in each case, and used to compare subfields between AD and controls. The magnitudes of R(2) and R(2)* changed very little between AD and control, but their variances in the Cornu Ammonis and dentate gyrus were significantly higher in AD compared for controls (p<0.001). To investigate the relationship between tissue iron and MRI parameters, each tissue block was cryosectioned at 30μm in the imaging plane, and iron distribution was mapped using synchrotron microfocus X-ray fluorescence spectroscopy. A positive correlation of R(2) and R(2)* with iron was demonstrated. While studies with fixed tissues are more straightforward to conduct, fixation can alter iron status in tissues, making measurement of unfixed tissue relevant. To our knowledge, these data represent an advance in quantitative imaging of hippocampal subfields in unfixed tissue, and the methods facilitate direct analysis of the relationship between MRI parameters and iron. The significantly increased variance in AD compared for controls warrants investigation at lower fields and in-vivo, to determine if this parameter is clinically relevant. |
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Authors:
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Vijay Antharam; Joanna F Collingwood; John-Paul Bullivant; Mark R Davidson; Saurav Chandra; Albina Mikhaylova; Mary Finnegan; Christopher Batich; John R Forder; Jon Dobson |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-8-16 |
Journal Detail:
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Title: NeuroImage Volume: - ISSN: 1095-9572 ISO Abbreviation: - Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-8-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9215515 Medline TA: Neuroimage Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier Inc. |
Affiliation:
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Department of Medicine, University of Florida, Gainesville, FL, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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