| High fatty acid availability after exercise alters the regulation of muscle lipid metabolism. | |
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MedLine Citation:
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PMID: 20870251 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We previously reported that a single exercise session protects against fatty acid (FA)-induced insulin resistance, perhaps in part through augmented intramyocellular triacylglycerol (IMTG) synthesis. The aim of this study was to examine the effect of elevated FA availability after exercise on factors regulating IMTG metabolism. After exercise (90 minutes, 65% peak oxygen uptake), 7 healthy women (body mass index, 23 ± 1 kg/m(2)) were infused overnight (16 hours) with either a lipid and heparin solution (LIPID, 0.11 g fat per kilogram per hour) or saline (SALINE). We measured resting FA oxidation (indirect calorimetry) and obtained a skeletal muscle biopsy sample the next morning. The 4-fold increase in overnight plasma FA concentration during LIPID increased IMTG by approximately 30% during LIPID vs SALINE. This was accompanied by an approximately 25% greater membrane-associated abundance of the FA transporter FAT/CD36 (P < .01) and an approximately 8% increase in the activity of the IMTG synthesis enzyme glycerol-3-phosphate acyltransferase (GPAT, P < .01). In contrast, resting FA oxidation was not affected. We also found no difference in the protein abundance of GPAT1 and diacylglycerol acyltransferase-1, diacylglycerol acyltransferase activity, or the abundance of the lipid droplet coat proteins (perilipins 2, 3, 4, and 5) between treatments. Our findings suggest that augmented capacity for FA flux into muscle (ie, via membrane-associated FAT/CD36), perhaps together with a slight yet significant increase in activity of a key IMTG synthesis enzyme (GPAT), may enhance IMTG storage when FA availability is high after exercise. The importance of the absence of a change in perilipin protein abundance despite increased muscle lipid storage remains to be determined. |
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Authors:
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Sean A Newsom; Simon Schenk; Minghua Li; Allison C Everett; Jeffrey F Horowitz |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-09-25 |
Journal Detail:
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Title: Metabolism: clinical and experimental Volume: 60 ISSN: 1532-8600 ISO Abbreviation: Metab. Clin. Exp. Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-05-17 Completed Date: 2011-07-19 Revised Date: 2013-01-29 |
Medline Journal Info:
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Nlm Unique ID: 0375267 Medline TA: Metabolism Country: United States |
Other Details:
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Languages: eng Pagination: 852-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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School of Kinesiology, University of Michigan, Ann Arbor, MI 48109, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Anaerobic Threshold / physiology Antigens, CD36 / metabolism Blotting, Western Carrier Proteins Ceramides / metabolism Diacylglycerol O-Acyltransferase / metabolism Exercise / physiology* Exercise Test Fats / metabolism Fatty Acids / metabolism* Female Glycerol-3-Phosphate O-Acyltransferase / metabolism Humans Lipid Metabolism / physiology* Muscle, Skeletal / enzymology, physiology* Oxidation-Reduction Phosphoproteins / metabolism Pulmonary Gas Exchange / physiology Triglycerides / biosynthesis |
| Grant Support | |
ID/Acronym/Agency:
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DK020572/DK/NIDDK NIH HHS; P30 DK089503/DK/NIDDK NIH HHS; P30-DK089503/DK/NIDDK NIH HHS; R01 DK071955/DK/NIDDK NIH HHS; R01 DK071955/DK/NIDDK NIH HHS; R01 DK071955-05/DK/NIDDK NIH HHS; R01 DK077966/DK/NIDDK NIH HHS; UL1RR024986/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD36; 0/Carrier Proteins; 0/Ceramides; 0/Fats; 0/Fatty Acids; 0/Phosphoproteins; 0/Triglycerides; 0/perilipin 1; EC 2.3.1.15/Glycerol-3-Phosphate O-Acyltransferase; EC 2.3.1.20/Diacylglycerol O-Acyltransferase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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