Document Detail


High-fat feeding induces tissue-specific alteration in proportion of activated insulin receptors in rats.
MedLine Citation:
PMID:  1691570     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
High dietary fat intake causes glucose intolerance and insulin resistance in man and in laboratory rats. We studied possible mechanisms of this insulin resistance in rat kidney, muscle and liver. In high-fat fed rats the body weight, plasma insulin concentration, plasma glucose levels, and serum triglyceride concentration were significantly higher than in the control rats. 125I-insulin binding to kidney basolateral membrane insulin receptors from high-fat fed rats was lower than in control rats. Basal as well as insulin-stimulated tyrosine kinase activity per insulin receptor was higher in the high-fat fed group, accompanied by increased autophosphorylation of the beta-subunit of the receptor and higher proportion of tyrosine-phosphorylated insulin receptors. In contrast, both in the skeletal muscle and the liver the insulin-stimulated tyrosine kinase activity per insulin receptor was significantly lower in high-fat fed animals, accompanied by diminished autophosphorylation of the beta-subunit of the receptor and lower proportion of tyrosine-phosphorylated receptors. Our results indicate tissue-specific alterations in transmembrane signaling induced by high-fat feeding in target tissues for insulin which in turn might contribute to the observed insulin resistance.
Authors:
K Nagy; J Levy; G Grunberger
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta endocrinologica     Volume:  122     ISSN:  0001-5598     ISO Abbreviation:  Acta Endocrinol.     Publication Date:  1990 Mar 
Date Detail:
Created Date:  1990-05-15     Completed Date:  1990-05-15     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0370312     Medline TA:  Acta Endocrinol (Copenh)     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  361-8     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Membrane / metabolism
Dietary Fats / administration & dosage,  pharmacology*
Insulin / metabolism
Insulin Resistance*
Kidney / metabolism*
Liver / metabolism*
Male
Muscles / metabolism*
Phosphorylation
Phosphotyrosine
Protein-Tyrosine Kinases / metabolism
Rats
Rats, Inbred Strains
Receptor, Insulin / metabolism*
Tyrosine / analogs & derivatives,  metabolism
Chemical
Reg. No./Substance:
0/Dietary Fats; 11061-68-0/Insulin; 21820-51-9/Phosphotyrosine; 55520-40-6/Tyrosine; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 2.7.10.1/Receptor, Insulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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