Document Detail

High fat diets and mouse colon mucosal membranes: a centrifugation study.
MedLine Citation:
PMID:  6317166     Owner:  NLM     Status:  MEDLINE    
Female Swiss mice were fed on a control diet (laboratory chow, 6% fat) or high fat diets (laboratory chow with added fat, principally corn oil, to produce a fat content of 16% or 23%). Homogenate of colon mucosa was fractionated by isopycnic banding on a linear sucrose gradient and the distribution of 5'-nucleotidase determined. Two main peaks were detected which we interpret as belonging to basolateral plasma membrane (BLPM) and brush borders. A high fat diet did not affect the relative sizes of the peaks or the median density of the BLPM peak but did significantly reduce the density of the brush border peak from 1.208 g/ml to 1.197 g/ml.
N J Temple; S M El-Khatib
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer letters     Volume:  21     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1984-02-23     Completed Date:  1984-02-23     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  225-31     Citation Subset:  IM    
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MeSH Terms
Cell Membrane / metabolism
Centrifugation, Density Gradient
Colon / metabolism*
Dietary Fats / administration & dosage*
Intestinal Mucosa / metabolism*
Microvilli / metabolism
Mucous Membrane / metabolism
Nucleotidases / analysis
Reg. No./Substance:
0/Dietary Fats; EC 3.1.3.-/Nucleotidases; EC'-Nucleotidase

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