| High-fat diet causes increased serum insulin and glucose which synergistically lead to renal tubular lipid deposition and extracellular matrix accumulation. | |
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MedLine Citation:
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PMID: 21733321 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Renal tubular lipid accumulation is associated with renal injury in the metabolic syndrome, but its mechanisms are not fully elucidated. The purpose of the present study was to investigate the exact mechanism of renal tubular lipid accumulation in the diet-induced metabolic syndrome. The in vivo experiments showed that a high-fat diet induced hyperglycaemia, hyperinsulinaemia and hypertriacylglycerolaemia, subsequent increases in sterol regulatory element binding protein-1 (SREBP-1) and transforming growth factor-β1 (TGF-β1), lipid droplet deposit in renal tubular cells and interstitial extracellular matrix accumulation in Wistar rats. A human renal proximal tubular epithelial cell line (HKC) was used to determine the direct role of insulin, and the results revealed that insulin induced SREBP-1, fatty acid synthase (FASN), TGF-β1 expressions, lipid droplet and extracellular matrix deposits. Knockdown of SREBP-1 by RNA interference technology significantly inhibited FASN, TGF-β1 up-regulation, lipid and extracellular matrix accumulation caused by insulin. In addition, we found that insulin and high glucose could synergistically increase SREBP-1, FASN, TGF-β1 and fibronectin expressions in HKC cells. These results indicate that high-fat diet-induced increased serum insulin and glucose synergistically cause renal tubular lipid deposit and extracellular matrix accumulation via the SREBP-1 pathway. |
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Authors:
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Jun Hao; Shu-Xia Liu; Song Zhao; Qing-Juan Liu; Wei Liu; Hui-Jun Duan |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-6-28 |
Journal Detail:
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Title: The British journal of nutrition Volume: - ISSN: 1475-2662 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-7-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372547 Medline TA: Br J Nutr Country: - |
Other Details:
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Languages: ENG Pagination: 1-12 Citation Subset: - |
Affiliation:
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Department of Pathology, Hebei Medical University, NO361 Zhongshan East Road, Shijiazhuang City, Hebei Province, People's Republic of China. |
Export Citation:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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