| High-fat diet based on dried bovine brain: an effective animal model of dyslipidemia and insulin resistance. | |
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MedLine Citation:
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PMID: 21437730 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Currently, there are no reports in the literature demonstrating any animal model that ingests one of the fattiest animal food source, the bovine brain. We hypothesized that a high-fat diet (HFD), based on dried bovine brain, could be used to develop an animal model possessing a spectrum of insulin resistance-related features. The HFD was formulated with 40% dried bovine brain plus 16.4% butter fat, prepared in-house. Furthermore, the diet contained 52% calories as fat and 73% of total fatty acids were saturated. Swiss mice weighing about 40 g were assigned to two dietary groups (n = 6/group), one group received a standard chow diet and the other was given HFD for 3 months. The body weight and biochemical parameters of the animals were measured initially and at monthly intervals until the end of the experiment. Animals fed on a HFD showed a significant increase in the body and adipose tissue weight, serum total cholesterol and triglyceride levels, when compared with mice fed on the control diet. Additionally, the HFD group showed higher circulating levels of liver transaminases, such as alanine aminotransferase and aspartate aminotransferase, compared with the control group. Finally, to illustrate the usefulness of this model, we report that the HFD induced mild hyperglycemia, fasting hyperinsulinemia, and increased the homeostasis model of assessment (HOMA-IR), in comparison with the control group. In conclusion, our results show that HFD, based on dried bovine brain, causes insulin resistance-related metabolic disturbances. Thus, this may be a suitable model to study disturbances in energy metabolism and their consequences. |
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Authors:
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Tiago Gomes Araújo; Ana Catarina Rezende Leite; Caíque Silveira Martins da Fonseca; Bruno Melo Carvalho; Alexandre Ricardo Pereira Schuler; Vera Lúcia de Menezes Lima |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-25 |
Journal Detail:
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Title: Journal of physiology and biochemistry Volume: - ISSN: 1877-8755 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-3-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9812509 Medline TA: J Physiol Biochem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Departamento de Bioquímica - Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, S/N, Cidade Universitária, 50670-901, Recife, PE, Brazil. |
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