Document Detail


High expression and nuclear localization of β-catenin in diffuse large B-cell lymphoma.
MedLine Citation:
PMID:  22427053     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Wnt/β-catenin signaling pathway plays diverse roles in embryonic development and maintenance of organs and tissues in adults as well as in the pathogenesis of a range of diseases, including many types of carcinomas. β-catenin, the principal downstream effector of the Wnt/β-catenin pathway, migrates to the nucleus and mediates the activation of the Wnt/β-catenin pathway. The aim of the present study was to investigate the expression and localization of β-catenin in diffuse large B-cell lymphoma (DLBCL) tissues and to illuminate the role of β-catenin in the pathogenesis of DLBCL. The mRNA expression levels of β-catenin were determined by quantitative polymerase chain reaction (PCR), while β-catenin protein levels were detected by western blot analysis and immunohistochemical staining. DLBCL showed a higher expression of β-catenin in contrast to reactive hyperplasia of lymph node tissues at both the mRNA and protein levels (P<0.001). Immunohistochemical analysis indicated nuclear localization of β-catenin in 14 (46.67%) DLBCL cases, whereas no inflammatory lymph node tissue showed nuclear accentuation of β-catenin. The overexpression and nuclear accentuation of β-catenin were strongly correlated to the clinical staging of patients with DLBCL (P<0.05). These results suggest that the Wnt/β-catenin pathway is partly activated in DLBCL and may contribute to its pathogenesis.
Authors:
Xueling Ge; Xiao Lv; Lili Feng; Xiaoqian Liu; Xin Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-03-13
Journal Detail:
Title:  Molecular medicine reports     Volume:  5     ISSN:  1791-3004     ISO Abbreviation:  Mol Med Rep     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-04-16     Completed Date:  2012-08-07     Revised Date:  2013-02-22    
Medline Journal Info:
Nlm Unique ID:  101475259     Medline TA:  Mol Med Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1433-7     Citation Subset:  IM    
Affiliation:
Department of Hematology, Shandong University, Jinan, Shandong, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Aged
Cell Nucleus / metabolism*
Female
Gene Expression Regulation, Neoplastic*
Humans
Immunohistochemistry
Lymphoma, Large B-Cell, Diffuse / metabolism*,  pathology
Male
Middle Aged
Neoplasm Staging
RNA, Messenger / metabolism
Up-Regulation
Wnt Signaling Pathway
beta Catenin / analysis,  genetics,  metabolism*
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/beta Catenin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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