Document Detail


High expression of integrin beta1 correlates with high proliferation capacity in oral keratinocytes.
MedLine Citation:
PMID:  17571237     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Large defects of the oral mucosa are still a major challenge in reconstructive surgery. For the development of oral mucosal grafts, successful enrichment of cells with a high proliferative potential is highly desirable. Therefore, the aim of this study was to separate two fractions of oral keratinocytes based on their affinity to collagen type IV. BASIC RESEARCH DESIGN: Oral keratinocytes were isolated from oral mucosa and separated into two fractions with different affinities to collagen type IV. Growth curves, Western blot analysis and immunohistochemical staining were used to detect differences between the two cell fractions. RESULTS: The cell fraction (RAC-IV) that adhered to collagen type IV within 20 min showed higher proliferative potential, significantly higher (P < 0.05) expression of integrin beta1 and fewer apoptotic cells. In particular, this fraction included small proliferating cells with the typical polygonal shape of oral keratinocytes, whereas the non-proliferating cells (RAC-IV-D) were irregularly shaped. Immunohistochemical staining showed only some apoptotic RAC-IV cells, whereas RAC-IV-D cells showed a significant increase of M30-positive cells. In addition, Western blotting revealed significantly higher (P < 0.05) expression of integrin beta1 in the RAC-IV fraction than in the RAC-IV-D fraction. CONCLUSION: Our results show that it is possible to enrich a fraction of highly proliferative oral keratinocytes by means of their high affinity to collagen type IV.
Authors:
Elisabeth Stein; Karin Blaimauer; Sieglinde Bauer; Boban M Erovic; Dritan Turhani; Dietmar Thurnher
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Wiener klinische Wochenschrift     Volume:  119     ISSN:  0043-5325     ISO Abbreviation:  Wien. Klin. Wochenschr.     Publication Date:  2007  
Date Detail:
Created Date:  2007-06-15     Completed Date:  2007-11-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  21620870R     Medline TA:  Wien Klin Wochenschr     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  318-22     Citation Subset:  IM    
Affiliation:
Department of Otorhinolaryngology, Head and Neck Surgery, Medical University of Vienna, Vienna, Austria.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Antigens, CD29 / analysis*
Blotting, Western
Cell Count
Cell Fractionation
Cell Proliferation
Cells, Cultured
Child
Child, Preschool
Collagen Type IV / metabolism
Humans
Immunohistochemistry
Keratinocytes / cytology*,  metabolism
Middle Aged
Mouth Mucosa / cytology*,  transplantation
Staining and Labeling
Time Factors
Tissue Engineering*
Chemical
Reg. No./Substance:
0/Antigens, CD29; 0/Collagen Type IV

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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