Document Detail


High expression of antizyme inhibitor 2, an activator of ornithine decarboxylase in steroidogenic cells of human gonads.
MedLine Citation:
PMID:  19756694     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
High activity of ornithine decarboxylase (ODC), the rate-limiting enzyme of polyamine synthesis, is typically present in rapidly proliferating normal and malignant cells. The mitotically inactive steroidogenic cells in rodent testis and ovaries, however, also display high ODC activity. The activity of ODC in these cells responds to luteinizing hormone, and inhibition of ODC reduces the production of steroid hormones. Polyamines and ODC also control proliferation of germ cells and spermiogenesis. The activity of ODC, especially in proliferating cells, is regulated by antizyme inhibitor (AZIN). This protein displaces ODC from a complex with its inhibitor, antizyme. We have previously identified and cloned a second AZIN, i.e. antizyme inhibitor 2 (AZIN2), which has the highest levels of expression in brain and in testis. In the present study, we have used immunohistochemistry and in situ hybridization to localize the expression of AZIN2 in human gonads. We found a robust expression of AZIN2 in steroidogenic cells: testicular Leydig cells and Leydig cell tumors, in ovarian luteinized cells lining corpus luteum cysts, and in hilus cells. The results suggest that AZIN2 is not primarily involved in regulating the proliferation of the germinal epithelium, indicating a different role for AZIN1 and AZIN2 in the regulation of ODC. The localization of AZIN2 implies possible involvement in the gonadal synthesis and/or release of steroid hormones.
Authors:
Laura T Mäkitie; Kristiina Kanerva; Anna Sankila; Leif C Andersson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-16
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  132     ISSN:  1432-119X     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2010-01-21     Completed Date:  2010-04-15     Revised Date:  2011-07-20    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  633-8     Citation Subset:  IM    
Affiliation:
Department of Pathology, Haartman Institute, University of Helsinki, P.O. Box 21, 00014, Helsinki, Finland.
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MeSH Terms
Descriptor/Qualifier:
Carrier Proteins / analysis,  genetics*
Female
Gonads / metabolism*
Humans
Immunohistochemistry
In Situ Hybridization
Leydig Cell Tumor / chemistry
Leydig Cells / chemistry
Male
Ornithine Decarboxylase / metabolism*
Ovarian Cysts / chemistry
Steroids / biosynthesis*
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Steroids; EC 4.1.1.17/Ornithine Decarboxylase; EC 4.1.1.19/ADC protein, human

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