| High expression of AID and active class switch recombination might account for a more aggressive disease in unmutated CLL patients: link with an activated microenvironment in CLL disease. | |
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MedLine Citation:
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PMID: 20233972 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Interaction of chronic lymphocytic leukemia (CLL) B cells with tissue microenvironment has been suggested to favor disease progression by promoting malignant B-cell growth. Previous work has shown expression in peripheral blood (PB) of CLL B cells of activation-induced cytidine deaminase (AID) among CLL patients with an unmutated (UM) profile of immunoglobulin genes and with ongoing class switch recombination (CSR) process. Because AID expression results from interaction with activated tissue microenvironment, we speculated whether the small subset with ongoing CSR is responsible for high levels of AID expression and could be derived from this particular microenvironment. In this work, we quantified AID expression and ongoing CSR in PB of 50 CLL patients and characterized the expression of different molecules related to microenvironment interaction. Our results show that among UM patients (1) high AID expression is restricted to the subpopulation of tumoral cells ongoing CSR; (2) this small subset expresses high levels of proliferation, antiapoptotic and progression markers (Ki-67, c-myc, Bcl-2, CD49d, and CCL3/4 chemokines). Overall, this work outlines the importance of a cellular subset in PB of UM CLL patients with a poor clinical outcome, high AID levels, and ongoing CSR, whose presence might be a hallmark of a recent contact with the microenvironment. |
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Authors:
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Florencia Palacios; Pilar Moreno; Pablo Morande; Cecilia Abreu; Agust?n Correa; Valentina Porro; Ana Ines Landoni; Raul Gabus; Mirta Giordano; Guillermo Dighiero; Otto Pritsch; Pablo Oppezzo |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-16 |
Journal Detail:
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Title: Blood Volume: 115 ISSN: 1528-0020 ISO Abbreviation: Blood Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-04 Completed Date: 2010-06-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7603509 Medline TA: Blood Country: United States |
Other Details:
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Languages: eng Pagination: 4488-96 Citation Subset: AIM; IM |
Affiliation:
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Unit of Recombinant Protein, Institut Pasteur de Montevideo, Montevideo, Uruguay. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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B-Lymphocyte Subsets
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enzymology,
immunology,
pathology Base Sequence Cell Proliferation Cytidine Deaminase / blood*, genetics* DNA Primers / genetics Gene Expression Humans Immunoglobulin Class Switching* Leukemia, Lymphocytic, Chronic, B-Cell / enzymology*, genetics*, immunology Mutation Prognosis RNA, Messenger / blood, genetics RNA, Neoplasm / blood, genetics Tumor Markers, Biological / genetics |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/RNA, Messenger; 0/RNA, Neoplasm; 0/Tumor Markers, Biological; EC 3.5.4.-/AICDA (activation-induced cytidine deaminase); EC 3.5.4.5/Cytidine Deaminase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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