Document Detail

High energy shock waves (HESW) for sonodynamic therapy: effects on HT-29 human colon cancer cells.
MedLine Citation:
PMID:  17094450     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Whether high energy shock waves (HESW), generated by a piezoelectric generator, were able to activate a sonosensitizer, 5-aminolevulinic acid (ALA) and induce inhibition of cell growth in HT-29 human colorectal cancer cells was investigated. MATERIALS AND METHODS: Cell survival and cell death pathways were investigated by cell growth curves, flow cytometry analysis and ELISA nucleosome evaluation. HT-29 cells were exposed to ALA and different HESW treatments: E1 (energy flux density = 0.22 mJ/mm2; 500 and 1000 shots) and E2 (energy flux density = 0.88 mJ/mm2; 500 and 1000 shots). RESULTS: A significant reduction of HT-29 cell growth with respect to untreated cells was observed only after treatment with ALA and HESW E2, 500 shots. In particular, HESWE2, 500 shots, was able to induce an arrest of HT-29 cells exposed to ALA in the G0/G1-phase of the cell cycle. CONCLUSION: HESW is proposed for the sonodynamic treatment of cancer cells.
Roberto Canaparo; Loredana Serpe; Maria Graziella Catalano; Ornella Bosco; Gian Paolo Zara; Laura Berta; Roberto Frairia
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  26     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    2006 Sep-Oct
Date Detail:
Created Date:  2006-11-10     Completed Date:  2006-11-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  3337-42     Citation Subset:  IM    
Department of Clinical Pathophysiology, University of Torino, Via Genova 3, 10126 Torino, Italy.
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MeSH Terms
Aminolevulinic Acid / pharmacology*
Cell Proliferation / drug effects,  radiation effects*
Cell Survival / drug effects,  radiation effects*
Combined Modality Therapy
Enzyme-Linked Immunosorbent Assay
HT29 Cells / drug effects,  radiation effects
High-Energy Shock Waves / therapeutic use*
Nucleosomes / metabolism
Reactive Oxygen Species / metabolism
Ultrasonic Therapy*
Reg. No./Substance:
0/Nucleosomes; 0/Reactive Oxygen Species; 106-60-5/Aminolevulinic Acid

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