Document Detail


High-energy phosphates in quiescent, beating and contracted cardiac cells.
MedLine Citation:
PMID:  3260516     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Isolated myocytes from ventricles are quiescent in the presence of 0.9 mM calcium. However, it is possible to induce beating by adding 0.5 mM BaCl2 to the media or to induce a contracture by elevating the external concentration of potassium (72.5 mM K). During the viable stage of contracture, which is up to 1 h, the sarcomere spacing is 1.7 +/- 0.1 micron and no leakage of intracellular components is observed. The metabolic properties of the cells in quiescent, beating and contracted states were compared. The O2 consumption (natom per mg cell protein per min) increased from 10-11 in quiescent cells to 60-66 in beating cells and 90-99 in contractured cells. In contrast no significant difference was found in the metabolite levels in the three cellular states: (nmol per mg cell protein +/- S.E.M.) ATP, 20.9 +/- 1.5; CrP, 22.3 +/- 2.2; ADP, 6.03 +/- 0.67; Cr, 10.8 +/- 1. It is proposed that the combined action of myosine ATPase, ATP synthase and cytosolic and mitochondrial creatine kinases serves to buffer the metabolite levels during periods of enhanced oxygen consumption.
Authors:
M Arrio-Dupont; D de Nay
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  934     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1988 Jul 
Date Detail:
Created Date:  1988-08-19     Completed Date:  1988-08-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  264-8     Citation Subset:  IM    
Affiliation:
Laboratoire de Physiologie Cellulaire Cardiaque, INSERM U-241, Université de Paris-Sud, Orsay, France.
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MeSH Terms
Descriptor/Qualifier:
Adenine Nucleotides / metabolism*
Animals
Creatine / metabolism
Culture Media
Myocardial Contraction*
Myocardium / cytology,  metabolism*
Oxygen Consumption
Phosphocreatine / metabolism*
Potassium / physiology
Rana esculenta
Sodium / physiology
Chemical
Reg. No./Substance:
0/Adenine Nucleotides; 0/Culture Media; 57-00-1/Creatine; 67-07-2/Phosphocreatine; 7440-09-7/Potassium; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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