Document Detail

High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier.
MedLine Citation:
PMID:  2692852     Owner:  NLM     Status:  MEDLINE    
A method, using LiAc to yield competent cells, is described that increased the efficiency of genetic transformation of intact cells of Saccharomyces cerevisiae to more than 1 X 10(5) transformants per microgram of vector DNA and to 1.5% transformants per viable cell. The use of single stranded, or heat denaturated double stranded, nucleic acids as carrier resulted in about a 100 fold higher frequency of transformation with plasmids containing the 2 microns origin of replication. Single stranded DNA seems to be responsible for the effect since M13 single stranded DNA, as well as RNA, was effective. Boiled carrier DNA did not yield any increased transformation efficiency using spheroplast formation to induce DNA uptake, indicating a difference in the mechanism of transformation with the two methods.
R H Schiestl; R D Gietz
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Current genetics     Volume:  16     ISSN:  0172-8083     ISO Abbreviation:  Curr. Genet.     Publication Date:  1989 Dec 
Date Detail:
Created Date:  1990-03-15     Completed Date:  1990-03-15     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8004904     Medline TA:  Curr Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  339-46     Citation Subset:  IM    
Department of Biology, University of Rochester, NY 14627.
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MeSH Terms
DNA, Fungal / genetics,  metabolism
DNA, Single-Stranded / genetics,  metabolism*
Hot Temperature
Nucleic Acid Denaturation
RNA, Fungal / metabolism
Saccharomyces cerevisiae / genetics*
Spheroplasts / metabolism
Time Factors
Transformation, Genetic*
Grant Support
Reg. No./Substance:
0/DNA, Fungal; 0/DNA, Single-Stranded; 0/RNA, Fungal

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