Document Detail

High efficiency transfection of primary skeletal muscle cells with lipid-based reagents.
MedLine Citation:
PMID:  12115959     Owner:  NLM     Status:  MEDLINE    
Lipofection is a convenient method for gene transfer into muscle cells but reportedly is inefficient. We tested the efficacy of commercially available lipid-based and polyamine transfection reagents. Primary rat skeletal muscle cell cultures were transfected at three stages of development and assayed after fusion. Efficiency reached 30% during the proliferation stage and up to 23% when most myoblasts had fused into myotubes. Optimization of transfection conditions with three different vectors yielded efficiencies exceeding 50%. Thus, lipid-based transfection into primary skeletal muscle cells can be several times more efficient than previously reported.
Birgit Neuhuber; David I Huang; Mathew P Daniels; Carol E Torgan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Muscle & nerve     Volume:  26     ISSN:  0148-639X     ISO Abbreviation:  Muscle Nerve     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-07-12     Completed Date:  2002-08-05     Revised Date:  2006-04-21    
Medline Journal Info:
Nlm Unique ID:  7803146     Medline TA:  Muscle Nerve     Country:  United States    
Other Details:
Languages:  eng     Pagination:  136-40     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Wiley Periodicals, Inc.
Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, 50 South Drive, Bethesda, Maryland 20892-8017, USA.
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MeSH Terms
Cells, Cultured
Chloramphenicol O-Acetyltransferase / genetics
DNA / genetics,  metabolism*
Feasibility Studies
Gene Expression / drug effects
Genes, Reporter
Genetic Vectors
Green Fluorescent Proteins
Indicators and Reagents / pharmacology*
Lipids / pharmacology*
Luminescent Proteins / genetics
Muscle, Skeletal / cytology,  drug effects*,  metabolism
Transfection / methods*
beta-Galactosidase / genetics
Reg. No./Substance:
0/FuGene; 0/Indicators and Reagents; 0/Lipids; 0/Luminescent Proteins; 147336-22-9/Green Fluorescent Proteins; 9007-49-2/DNA; EC O-Acetyltransferase; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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