| High efficiency site-specific genetic engineering of the mosquito genome. | |
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MedLine Citation:
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PMID: 16640723 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Current techniques for the genetic engineering of insect genomes utilize transposable genetic elements, which are inefficient, have limited carrying capacity and give rise to position effects and insertional mutagenesis. As an alternative, we investigated two site-specific integration mechanisms in the yellow fever mosquito, Aedes aegypti. One was a modified CRE/lox system from phage P1 and the other a viral integrase system from Streptomyces phage phi C31. The modified CRE/lox system consistently failed to produce stable germline transformants but the phi C31 system was highly successful, increasing integration efficiency by up to 7.9-fold. The ability to efficiently target transgenes to specific chromosomal locations and the potential to integrate very large transgenes has broad applicability to research on many medically and economically important species. |
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Authors:
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D D Nimmo; L Alphey; J M Meredith; P Eggleston |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Insect molecular biology Volume: 15 ISSN: 0962-1075 ISO Abbreviation: Insect Mol. Biol. Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-04-27 Completed Date: 2006-08-21 Revised Date: 2013-03-26 |
Medline Journal Info:
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Nlm Unique ID: 9303579 Medline TA: Insect Mol Biol Country: England |
Other Details:
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Languages: eng Pagination: 129-36 Citation Subset: IM |
Affiliation:
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Centre for Applied Entomology and Parasitology, Keele University, Keele, Staffordshire UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aedes
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genetics* Animals Bacteriophages Gene Targeting Genetic Engineering / methods* Genetic Vectors Genome, Insect* Integrases Transduction, Genetic Viral Proteins Virus Integration |
| Grant Support | |
ID/Acronym/Agency:
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056146//Wellcome Trust; 069162//Wellcome Trust; 077593//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Viral Proteins; EC 2.7.7.-/Cre recombinase; EC 2.7.7.-/Integrases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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