Document Detail


High-efficiency derivation of human embryonic stem cell lines following pre-implantation genetic diagnosis.
MedLine Citation:
PMID:  20217271     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pre-implantation genetic diagnosis allows the characterisation of embryos that carry a gene responsible for a severe monogenic disease and to transfer to the mother's uterus only the unaffected one(s). The genetically affected embryos can be used to establish human embryonic stem cell (hESC) lines. We are currently establishing a cell bank of ESC lines carrying specific disease-causing mutant genes. These cell lines are available to the scientific community. For this purpose, we have designed a technique that requires only minimal manipulation of the embryos. At the blastocyst stage, we just removed the zona pellucida before seeding the embryo as a whole on a layer of feeder cells. This approach gave a good success rate (>20%), whatever the quality of the embryos, and allowed us to derive 11 new hESC lines, representing seven different pathologies. Full phenotypic validation of the cell lines according to ISCI guidelines confirmed their pluripotent nature, as they were positive for hESC markers and able to differentiate in vitro in all three germ layers derivatives. Nine out of 11 stem cell lines had normal karyotypes. Our results indicate that inner cell mass isolation is not mandatory for hESC derivation and that minimal manipulation of embryos can lead to high success rate.
Authors:
Philippe Tropel; Johana Tournois; Julien Côme; Christine Varela; Céline Moutou; Pascal Fragner; Michel Cailleret; Yacine Laâbi; Marc Peschanski; Stéphane Viville
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-09
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  46     ISSN:  1543-706X     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-19     Completed Date:  2010-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  376-85     Citation Subset:  IM    
Affiliation:
IGBMC, Illkirch 67404, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Surface / metabolism
Biological Markers / metabolism
Blastocyst / cytology*
Cell Culture Techniques / methods*
Cell Differentiation / genetics
Cell Line
Embryonic Stem Cells / cytology*
Female
Gene Expression Regulation, Developmental
Humans
Karyotyping
Male
Mice
Pedigree
Pluripotent Stem Cells / cytology,  metabolism
Preimplantation Diagnosis / methods*
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/Antigens, Surface; 0/Biological Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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