| High-efficiency derivation of human embryonic stem cell lines following pre-implantation genetic diagnosis. | |
| | |
MedLine Citation:
|
PMID: 20217271 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Pre-implantation genetic diagnosis allows the characterisation of embryos that carry a gene responsible for a severe monogenic disease and to transfer to the mother's uterus only the unaffected one(s). The genetically affected embryos can be used to establish human embryonic stem cell (hESC) lines. We are currently establishing a cell bank of ESC lines carrying specific disease-causing mutant genes. These cell lines are available to the scientific community. For this purpose, we have designed a technique that requires only minimal manipulation of the embryos. At the blastocyst stage, we just removed the zona pellucida before seeding the embryo as a whole on a layer of feeder cells. This approach gave a good success rate (>20%), whatever the quality of the embryos, and allowed us to derive 11 new hESC lines, representing seven different pathologies. Full phenotypic validation of the cell lines according to ISCI guidelines confirmed their pluripotent nature, as they were positive for hESC markers and able to differentiate in vitro in all three germ layers derivatives. Nine out of 11 stem cell lines had normal karyotypes. Our results indicate that inner cell mass isolation is not mandatory for hESC derivation and that minimal manipulation of embryos can lead to high success rate. |
| | |
Authors:
|
Philippe Tropel; Johana Tournois; Julien Côme; Christine Varela; Céline Moutou; Pascal Fragner; Michel Cailleret; Yacine Laâbi; Marc Peschanski; Stéphane Viville |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-09 |
Journal Detail:
|
Title: In vitro cellular & developmental biology. Animal Volume: 46 ISSN: 1543-706X ISO Abbreviation: In Vitro Cell. Dev. Biol. Anim. Publication Date: 2010 Apr |
Date Detail:
|
Created Date: 2010-04-19 Completed Date: 2010-07-08 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9418515 Medline TA: In Vitro Cell Dev Biol Anim Country: Germany |
Other Details:
|
Languages: eng Pagination: 376-85 Citation Subset: IM |
Affiliation:
|
IGBMC, Illkirch 67404, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antigens, Surface / metabolism Biological Markers / metabolism Blastocyst / cytology* Cell Culture Techniques / methods* Cell Differentiation / genetics Cell Line Embryonic Stem Cells / cytology* Female Gene Expression Regulation, Developmental Humans Karyotyping Male Mice Pedigree Pluripotent Stem Cells / cytology, metabolism Preimplantation Diagnosis / methods* Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, Surface; 0/Biological Markers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: From the patient's perspective: the impact of training on resident physician's obesity counseling.
Next Document: Effects of fluoride on synaptic membrane fluidity and PSD-95 expression level in rat hippocampus.