| High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells. | |
| | |
MedLine Citation:
|
PMID: 10477258 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki Forest virus (SFV) vectors allow high-efficiency expression of heterologous protein in chromaffin cells. |
| | |
Authors:
|
R R Duncan; A C Don-Wauchope; S Tapechum; M J Shipston; R H Chow; P Estibeiro |
Related Documents
:
|
12056638 - Effect of calcitriol on proliferation of tt cells and on expression of calcitonin gene. 15182418 - Mechanisms for hypoxia detection in o2-sensitive cells. 18685958 - Morphological changes in the salivary glands of amblyomma cajennense females (acari: ix... 11740048 - Differential reactivity for galectin-3 in hürthle cell adenomas and carcinomas. 2160288 - Significance of dinucleoside tetraphosphate production by cultured tumor cells exposed ... 22686188 - Different effects on cell proliferation and migration abilities of endothelial cells by... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Biochemical journal Volume: 342 Pt 3 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1999 Sep |
Date Detail:
|
Created Date: 1999-12-08 Completed Date: 1999-12-08 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 497-501 Citation Subset: IM |
Affiliation:
|
Membrane Biology Group, Biomedical Sciences, University of Edinburgh Medical School, Teviot Place, Edinburgh, EH8 9XD, U.K. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adrenal Medulla
/
cytology* Animals Cattle Chromaffin Cells / cytology, virology* Culture Media Electrophysiology Exocytosis Gene Transfer Techniques* Green Fluorescent Proteins Luminescent Proteins / genetics, metabolism Mice Microscopy, Confocal Polymerase Chain Reaction Semliki forest virus / genetics* |
| Grant Support | |
ID/Acronym/Agency:
|
//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media; 0/Luminescent Proteins; 147336-22-9/Green Fluorescent Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Repression of gene expression by oxidative stress.
Next Document: Salicylate-enhanced activation of transcription factors induced by interferon-gamma.