Document Detail

High density of long dinucleotide microsatellites in Drosophila subobscura.
MedLine Citation:
PMID:  10908646     Owner:  NLM     Status:  MEDLINE    
We isolated 96 dinucleotide repeats with five or more tandemly repeated units from a subgenomic Drosophila subobscura library. The mean repeat unit length of microsatellite clones in D. subobscura is 15, higher than that observed in other Drosophila species. Population variation was assayed in 32-40 chromosomes from Barcelona, Spain, using 18 randomly chosen microsatellite loci. Positive correlation between measures of variation and perfect repeat length measures (mean size, most common, and longest allele) is consistent with a higher mutation rate in loci with longer repeat units. Levels of microsatellite variation measured as variance in repeat number and heterozygosity in D. subobscura were similar to those of Drosophila pseudoobscura and higher than those of Drosophila melanogaster and Drosophila simulans. Our data suggest that higher levels of microsatellite variation, and possibly density, in D. subobscura compared with D. melanogaster are due to both a higher average effective population and a higher intrinsic slippage rate in the former species.
M Pascual; M D Schug; C F Aquadro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular biology and evolution     Volume:  17     ISSN:  0737-4038     ISO Abbreviation:  Mol. Biol. Evol.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-09-08     Completed Date:  2000-09-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8501455     Medline TA:  Mol Biol Evol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1259-67     Citation Subset:  IM    
Department of Molecular Biology and Genetics, Cornell University, New York, NY, USA.
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MeSH Terms
Base Sequence
DNA / chemistry,  genetics
Dinucleotide Repeats / genetics*
Drosophila / genetics*
Drosophila melanogaster / genetics
Genetic Variation
Genomic Library
Microsatellite Repeats / genetics*
Molecular Sequence Data
Sequence Analysis, DNA
Species Specificity
Grant Support
Reg. No./Substance:

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