| High-density lipoproteins limit neutrophil-induced damage to the blood-brain barrier in vitro. | |
| | |
MedLine Citation:
|
PMID: 23299241 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Breakdown of the blood-brain barrier (BBB) is a key step associated with ischemic stroke and its increased permeability causes extravasation of plasma proteins and circulating leukocytes. Polymorphonuclear neutrophil (PMN) proteases may participate in BBB breakdown. We investigated the role of PMNs in ischemic conditions by testing their effects on a model of BBB in vitro, under oxygen-glucose deprivation (OGD) to mimic ischemia, supplemented or not with high-density lipoproteins (HDLs) to assess their potential protective effects. Human cerebral endothelial cells cultured on transwells were incubated for 4 hours under OGD conditions with or without PMNs and supplemented or not with HDLs or alpha-1 antitrypsin (AAT, an elastase inhibitor). The integrity of the BBB was then assessed and the effect of HDLs on PMN-induced proteolysis of extracellular matrix proteins was evaluated. The release of myeloperoxidase and matrix metalloproteinase 9 (MMP-9) by PMNs was quantified. Polymorphonuclear neutrophils significantly increased BBB permeability under OGD conditions via proteolysis of extracellular matrix proteins. This was associated with PMN degranulation. Addition of HDLs or AAT limited the proteolysis and associated increased permeability by inhibiting PMN activation. Our results suggest a deleterious, elastase-mediated role of activated PMNs under OGD conditions leading to BBB disruption that could be inhibited by HDLs.Journal of Cerebral Blood Flow & Metabolism advance online publication, 9 January 2013; doi:10.1038/jcbfm.2012.206. |
| | |
Authors:
|
Quoc Bao Dang; Bertrand Lapergue; Alexy Tran-Dinh; Devy Diallo; Juan-Antonio Moreno; Mikael Mazighi; Ignacio A Romero; Babette Weksler; Jean-Baptiste Michel; Pierre Amarenco; Olivier Meilhac |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2013-1-09 |
Journal Detail:
|
Title: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Volume: - ISSN: 1559-7016 ISO Abbreviation: J. Cereb. Blood Flow Metab. Publication Date: 2013 Jan |
Date Detail:
|
Created Date: 2013-1-9 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8112566 Medline TA: J Cereb Blood Flow Metab Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
INSERM UMR698, Paris, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Glutamatergic function in the resting awake human brain is supported by uniformly high oxidative ene...
Next Document: Neurovascular protection by targeting early blood-brain barrier disruption with neurotrophic factors...