Document Detail

High density culture of mammalian cells with dynamic perfusion based on on-line oxygen uptake rate measurements.
MedLine Citation:
PMID:  7765589     Owner:  NLM     Status:  MEDLINE    
In a continuous culture with cell retention the perfusion rate must be adjusted dynamically to meet the cellular demand. An automated mechanism of adjusting the perfusion rate based on real-time measurement of the metabolic load of the bioreactor is important in achieving a high cell concentration and maintaining high viability. We employed oxygen uptake rate (OUR) measurement as an on-line metabolic indicator of the physiological state of the cells in the bioreactor and adjusted the perfusion rate accordingly. Using an internal hollow fiber microfiltration system for total cell retention, a cell concentration of almost 10(8) cells/mL was achieved. Although some aggregates were formed during the cultivation, the viability remained high as examined with confocal microscopy after fluorescent vital staining. The results demonstrate that on-line OUR measurement facilitates automated dynamic perfusion and allows a high cell concentration to be achieved.
Y S Kyung; M V Peshwa; D M Gryte; W S Hu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytotechnology     Volume:  14     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  1994  
Date Detail:
Created Date:  1995-01-10     Completed Date:  1995-01-10     Revised Date:  2008-10-09    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  183-90     Citation Subset:  B    
Department of Chemical Engineering & Materials Science, University of Minnesota, Minneapolis.
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MeSH Terms
Cell Line / metabolism*
Cell Survival / physiology
Online Systems*
Oxygen / metabolism*
Reg. No./Substance:

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