| High density culture of mammalian cells with dynamic perfusion based on on-line oxygen uptake rate measurements. | |
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MedLine Citation:
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PMID: 7765589 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In a continuous culture with cell retention the perfusion rate must be adjusted dynamically to meet the cellular demand. An automated mechanism of adjusting the perfusion rate based on real-time measurement of the metabolic load of the bioreactor is important in achieving a high cell concentration and maintaining high viability. We employed oxygen uptake rate (OUR) measurement as an on-line metabolic indicator of the physiological state of the cells in the bioreactor and adjusted the perfusion rate accordingly. Using an internal hollow fiber microfiltration system for total cell retention, a cell concentration of almost 10(8) cells/mL was achieved. Although some aggregates were formed during the cultivation, the viability remained high as examined with confocal microscopy after fluorescent vital staining. The results demonstrate that on-line OUR measurement facilitates automated dynamic perfusion and allows a high cell concentration to be achieved. |
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Authors:
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Y S Kyung; M V Peshwa; D M Gryte; W S Hu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cytotechnology Volume: 14 ISSN: 0920-9069 ISO Abbreviation: Cytotechnology Publication Date: 1994 |
Date Detail:
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Created Date: 1995-01-10 Completed Date: 1995-01-10 Revised Date: 2008-10-09 |
Medline Journal Info:
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Nlm Unique ID: 8807027 Medline TA: Cytotechnology Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 183-90 Citation Subset: B |
Affiliation:
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Department of Chemical Engineering & Materials Science, University of Minnesota, Minneapolis. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line
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metabolism* Cell Survival / physiology Humans Online Systems* Oxygen / metabolism* Perfusion |
| Chemical | |
Reg. No./Substance:
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7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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