| High density culture of HeLa cells in a CelliGen perfusion system. | |
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MedLine Citation:
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PMID: 1368408 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A hollow fiber cartridge may be used in an extraneous recycle loop to facilitate perfusion operation of a stirred tank bioreactor. Retention of cells while removing waste products and replenishment with fresh nutrients allows higher than normal cell densities obtained in batch or continuous culture systems. This system successfully propagated HeLa cells to over 11 million viable cells per milliliter. Much higher perfusion rates (up to 4 vessel volumes per day) were necessary for high density culture of HeLa cells compared to BHK or a hybridoma cell line because of a much higher specific cellular metabolic rate. Cell specific glucose consumption rate, lactate production and ammonia production rates are several times higher for HeLa cells. Reproducible high cell densities and viabilities can be repeatedly obtained after harvest and dilution of a HeLa cell culture by partial drainage and reconstitution in the bioreactor. |
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Authors:
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Y Chen; T L LaPorte; S S Wang; J Shevitz |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cytotechnology Volume: 8 ISSN: 0920-9069 ISO Abbreviation: Cytotechnology Publication Date: 1992 |
Date Detail:
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Created Date: 1992-09-14 Completed Date: 1992-09-14 Revised Date: 2008-10-09 |
Medline Journal Info:
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Nlm Unique ID: 8807027 Medline TA: Cytotechnology Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 85-8 Citation Subset: B |
Affiliation:
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New Brunswick Scientific Co., Inc., Edison, NJ 08818. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Ammonia
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metabolism Cell Count Cell Division Cell Survival Glucose / metabolism Hela Cells / cytology* Humans Kinetics Lactates / metabolism Perfusion |
| Chemical | |
Reg. No./Substance:
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0/Lactates; 50-99-7/Glucose; 7664-41-7/Ammonia |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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