Document Detail

High density culture of HeLa cells in a CelliGen perfusion system.
MedLine Citation:
PMID:  1368408     Owner:  NLM     Status:  MEDLINE    
A hollow fiber cartridge may be used in an extraneous recycle loop to facilitate perfusion operation of a stirred tank bioreactor. Retention of cells while removing waste products and replenishment with fresh nutrients allows higher than normal cell densities obtained in batch or continuous culture systems. This system successfully propagated HeLa cells to over 11 million viable cells per milliliter. Much higher perfusion rates (up to 4 vessel volumes per day) were necessary for high density culture of HeLa cells compared to BHK or a hybridoma cell line because of a much higher specific cellular metabolic rate. Cell specific glucose consumption rate, lactate production and ammonia production rates are several times higher for HeLa cells. Reproducible high cell densities and viabilities can be repeatedly obtained after harvest and dilution of a HeLa cell culture by partial drainage and reconstitution in the bioreactor.
Y Chen; T L LaPorte; S S Wang; J Shevitz
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytotechnology     Volume:  8     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  1992  
Date Detail:
Created Date:  1992-09-14     Completed Date:  1992-09-14     Revised Date:  2008-10-09    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  85-8     Citation Subset:  B    
New Brunswick Scientific Co., Inc., Edison, NJ 08818.
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MeSH Terms
Ammonia / metabolism
Cell Count
Cell Division
Cell Survival
Glucose / metabolism
Hela Cells / cytology*
Lactates / metabolism
Reg. No./Substance:
0/Lactates; 50-99-7/Glucose; 7664-41-7/Ammonia

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