Document Detail


High density cultivation of hybridoma in charged porous carriers.
MedLine Citation:
PMID:  7764959     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A porous carrier whose surface is charged with polyethyleneimine (PEI) was evaluated for high-density cultivation of immobilized hybridoma cells. The carrier was used either in suspension or in fixed bed. For suspended carriers, the effect of carrier pore size, ion-exchange capacity (IEC), mixing intensity and inoculum concentration on the attachment and growth of HBs MAb-producing hybridoma cells in carrier CELLSNOW EX were studied. The carrier with 100 microns pore size was more efficient than that with 500 microns in supporting cell attachment. In addition, the amount of antibody which was detected by PHA (passive hemagglutination assay) was 4-times higher in 100 microns compared with 500 microns pore size. At low initial cell concentration, 2.8 x 10(5) ml-1, the attached cells did not grow in the first 7 d as indicated by a negligible glucose consumption. However, cell proliferation was observed at high inoculum, 9 x 10(5) ml-1. Hybridoma cells were expected to attach faster to EX with high IEC than with low IEC but results showed no significant difference. A different hybridoma cell line producing monoclonal antibody (MAb) against penicillin-G-amidase was successfully cultivated in an axial-flow fixed bed containing CELLSNOW carriers. The specific glucose uptake rate as an indicator for cell activity was 24-times higher compared with conventional chemostat cultures. The CELLSNOW carriers offers many advantages over other carriers.
Authors:
C P Ong; R Pörtner; H Märkl; Y Yamazaki; K Yasuda; M Matsumura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biotechnology     Volume:  34     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  1994 May 
Date Detail:
Created Date:  1994-07-29     Completed Date:  1994-07-29     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  259-68     Citation Subset:  B    
Affiliation:
Biomaterial Co., Ltd., Fukui, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / biosynthesis
Biotechnology / instrumentation*
Cell Adhesion
Cell Count
Cell Division
Cytological Techniques / instrumentation*
Evaluation Studies as Topic
Glucose / metabolism
Hybridomas / cytology*,  immunology,  metabolism
Ion Exchange Resins
Mice
Particle Size
Polyethyleneimine
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Ion Exchange Resins; 50-99-7/Glucose; 9002-98-6/Polyethyleneimine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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