Document Detail


High concentrations of thiosulfate in scala tympani perilymph after systemic administration in the guinea pig.
MedLine Citation:
PMID:  18607994     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CONCLUSION: High concentrations of the antioxidant thiosulfate reach scala tympani perilymph after i.v. administration in the guinea pig. Thiosulfate concentrations in perilymph remain elevated longer than in blood. This warrants further studies on the possibility of obtaining otoprotection by thiosulfate administration several hours before that of cisplatin without compromising the anticancer effect caused by cisplatin inactivation in the blood compartment. OBJECTIVE: Thiosulfate may reduce cisplatin-induced ototoxicity, presumably by oxidative stress relief and formation of inactivate platinum complexes. This study aimed to explore to what extent thiosulfate reaches scala tympani perilymph after systemic administration in the guinea pig. MATERIALS AND METHODS: Scala tympani perilymph (1 microl) was aspirated from the basal turn of each cochlea up to 3 h after thiosulfate administration (103 mg/kg b.w., i.v.). Blood samples were also taken. Thiosulfate was quantified by HPLC and fluorescence detection. RESULTS: Substantial thiosulfate concentrations were found in perilymph. The area under the concentration-time curve for thiosulfate in perilymph and blood was 3100 microMxmin and 6300 microMxmin, respectively. The highest thiosulfate concentrations in perilymph were found at the first sampling at about 10 min. Due to a more rapid elimination from blood, perilymph concentrations exceeded those of blood towards the end of the experiment.
Authors:
Pernilla Videhult Pierre; Cecilia Engmér; Inger Wallin; Göran Laurell; Hans Ehrsson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta oto-laryngologica     Volume:  129     ISSN:  1651-2251     ISO Abbreviation:  Acta Otolaryngol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-19     Completed Date:  2009-04-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370354     Medline TA:  Acta Otolaryngol     Country:  Norway    
Other Details:
Languages:  eng     Pagination:  132-7     Citation Subset:  IM    
Affiliation:
Department of Oncology-Pathology, Karolinska Institutet, Karolinska University Hospital Solna, Stockholm, Sweden. pernilla.videhult-pierre@apoteket.se
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MeSH Terms
Descriptor/Qualifier:
Animals
Antioxidants / administration & dosage,  pharmacokinetics*
Biological Availability
Female
Guinea Pigs
Injections, Intravenous
Male
Metabolic Clearance Rate
Perilymph / metabolism*
Scala Tympani / metabolism*
Thiosulfates / administration & dosage,  pharmacokinetics*
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Thiosulfates; 7772-98-7/sodium thiosulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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