Document Detail

High concentrations of p-Glu-His-Pro-NH2 (thyrotropin-releasing hormone) occur in rat prostate.
MedLine Citation:
PMID:  6324143     Owner:  NLM     Status:  MEDLINE    
Thyrotropin-releasing hormone (TRH) immunoreactivity occurs in high concentration within the rat prostate. Previous studies have shown that the immunoreactive species consists of more than one TRH-like tripeptide which cross-reacts in the TRH radioimmunoassay. The component which was highly retained during cation exchange chromatography was subjected to a preparative scale isolation, purification and structural analysis. The methods used included methanol extraction, water-ethyl ether partitioning, cation exchange chromatography, affinity chromatography, high pressure liquid chromatography, TRH radioimmunoassay, in vitro pituitary bioassay, TRH receptor assay, and amino acid analysis. The mean concentration of the predominant amino acids (Glu, His, Pro), 344 pmoles/ml, and the TRH concentration measured by TRH radioimmunoassay prior to acid hydrolysis, 372 pmoles/ml, were nearly identical. Because the material analyzed cochromatographed with synthetic TRH in several chromatographic systems, had a radioreceptor potency which was indistinguishable from that for synthetic TRH, and released TSH and prolactin but not growth hormone from rat pituitaries in vitro, it is concluded that pGlu-His-Pro-NH2 is one of the TRH-like peptides in the rat vental prostate.
A E Pekary; B Sharp; J Briggs; H E Carlson; J M Hershman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Peptides     Volume:  4     ISSN:  0196-9781     ISO Abbreviation:  Peptides     Publication Date:    1983 Nov-Dec
Date Detail:
Created Date:  1984-05-11     Completed Date:  1984-05-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8008690     Medline TA:  Peptides     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  915-9     Citation Subset:  IM    
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MeSH Terms
Amino Acids / analysis
Biological Assay
Chromatography, High Pressure Liquid
Growth Hormone / secretion
Prolactin / secretion
Prostate / analysis*
Rats, Inbred Strains
Receptors, Cell Surface / metabolism
Receptors, Thyrotropin-Releasing Hormone
Thyrotropin / secretion
Thyrotropin-Releasing Hormone / analysis*,  metabolism,  physiology
Reg. No./Substance:
0/Amino Acids; 0/Receptors, Cell Surface; 0/Receptors, Thyrotropin-Releasing Hormone; 24305-27-9/Thyrotropin-Releasing Hormone; 9002-62-4/Prolactin; 9002-71-5/Thyrotropin; 9002-72-6/Growth Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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