Document Detail

High cell density cultivation of recombinant yeasts and bacteria under non-pressurized and pressurized conditions in stirred tank bioreactors.
MedLine Citation:
PMID:  17681630     Owner:  NLM     Status:  MEDLINE    
This study demonstrates the applicability of pressurized stirred tank bioreactors for oxygen transfer enhancement in aerobic cultivation processes. The specific power input and the reactor pressure was employed as process variable. As model organism Escherichia coli, Arxula adeninivorans, Saccharomyces cerevisiae and Corynebacterium glutamicum were cultivated to high cell densities. By applying specific power inputs of approx. 48kWm(-3) the oxygen transfer rate of a E. coli culture in the non-pressurized stirred tank bioreactor was lifted up to values of 0.51moll(-1)h(-1). When a reactor pressure up to 10bar was applied, the oxygen transfer rate of a pressurized stirred tank bioreactor was lifted up to values of 0.89moll(-1)h(-1). The non-pressurized stirred tank bioreactor was able to support non-oxygen limited growth of cell densities of more than 40gl(-1) cell dry weight (CDW) of E. coli, whereas the pressurized stirred tank bioreactor was able to support non-oxygen limited growth of cell densities up to 225gl(-1) CDW of A. adeninivorans, 89gl(-1) CDW of S. cerevisiae, 226gl(-1) CDW of C. glutamicum and 110gl(-1) CDW of E. coli. Compared to literature data, some of these cell densities are the highest values ever achieved in high cell density cultivation of microorganisms in stirred tank bioreactors. By comparing the specific power inputs as well as the k(L)a values of both systems, it is demonstrated that only the pressure is a scaleable tool for oxygen transfer enhancement in industrial stirred tank bioreactors. Furthermore, it was shown that increased carbon dioxide partial pressures did not remarkably inhibit the growth of the investigated model organisms.
Arnd Knoll; Stefan Bartsch; Bernward Husemann; Philip Engel; Kirsten Schroer; Betina Ribeiro; Christoph Stöckmann; Juri Seletzky; Jochen Büchs
Publication Detail:
Type:  Journal Article     Date:  2007-06-23
Journal Detail:
Title:  Journal of biotechnology     Volume:  132     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-10-26     Completed Date:  2007-12-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  167-79     Citation Subset:  IM    
Biochemical Engineering, RWTH Aachen University, 52056 Aachen, Germany.
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MeSH Terms
Bioreactors / microbiology*
Cell Count
Corynebacterium glutamicum / metabolism
Escherichia coli / metabolism
Fermentation / physiology*
Industrial Microbiology / methods*
Oxygen / metabolism*
Recombinant Proteins / metabolism
Saccharomyces cerevisiae / metabolism
Reg. No./Substance:
0/Recombinant Proteins; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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