Document Detail

High cell density cultivation and high recombinant protein production of Escherichia coli strain expressing uricase.
MedLine Citation:
PMID:  8611749     Owner:  NLM     Status:  MEDLINE    
Uricase from Cellulomonas flavigena SK-4 is an industrially useful enzyme for commercial formulations of hair coloring. The uricase production by recombinant Escherichia coli strain with a high cell density cultivation technique was described. Of three kinds of media, synthetic media with the feeding of a high concentration of glucose solution were suitable for high cell density cultivation. As for feeding, both biomass concentration and uricase productivity were increased by about two (61.2 g dry cell weight (DCW)/liter) and three times (1037 U/ml broth), respectively, in 24 h by continuous supply. In the case of feeding by a DO-stat method, however, cell concentration was comparable to continuous glucose supply but uricase activity was reduced. By supplying pure oxygen to compensate for oxygen limitation during cultivation, the highest values of 77.4g DCW/liter and 1113 U/ml broth of the uricase activity were achieved with the total cultivation time of 15 h.
S Nakagawa; H Oda; H Anazawa
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  59     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  1995 Dec 
Date Detail:
Created Date:  1996-06-06     Completed Date:  1996-06-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  2263-7     Citation Subset:  B    
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Japan.
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MeSH Terms
Actinomycetales / enzymology
Bacteriological Techniques
Escherichia coli / enzymology*,  genetics,  growth & development
Fungal Proteins / biosynthesis*,  genetics
Glucose / pharmacology
Oxygen / pharmacology
Recombinant Proteins / biosynthesis,  genetics
Urate Oxidase / biosynthesis*,  genetics,  metabolism
Reg. No./Substance:
0/Fungal Proteins; 0/Recombinant Proteins; 50-99-7/Glucose; 7782-44-7/Oxygen; EC Oxidase

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