Document Detail

High-affinity neurotensin receptor is involved in phosphoinositide hydrolysis stimulation by carbachol in neonatal rat brain.
MedLine Citation:
PMID:  15707678     Owner:  NLM     Status:  MEDLINE    
Ontogenetic studies indicate that inositol phosphate accumulation in rodent brain tissue by cholinergic muscarinic agonists as well as expression of high-affinity neurotensin receptor (NTS1) peak at 7 days after birth. Herein, potential participation of this receptor in such effect was investigated. Cerebral cortex prisms of 7-day-old rats were preloaded with [3H]myoinositol and later incubated during 60 or 20 min in the presence of muscarinic agonist carbachol plus neurotensin and SR 48692, a non-peptide NTS1 antagonist. In 60-min incubation experiments, inositol phosphate accumulation by 10(-3) M carbachol was roughly 320%, an effect which remained unaltered plus 10(-6) M to 10(-4) M neurotensin but partially decreased with equimolar SR 48692 concentration. In 20-min incubation experiments, inositol phosphate accumulation by 10(-3) M carbachol was circa 240%, a value which attained 320-360% plus 10(-7) M neurotensin; this effect was totally blocked by 10(-7) M SR 48692. It was concluded that in inositol phosphate accumulation by carbachol, besides the cholinergic muscarinic receptor, the NTS1 receptor is likewise involved; findings at 60 min are attributable to the effect of endogenous neurotensin whereas those at 20 min most likely involve both endogenous and exogenously added peptide.
S Pereyra-Alfonso; M G López Ordieres; M del V Armanino; G Rodríguez de Lores Arnaiz
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-01-11
Journal Detail:
Title:  Brain research. Developmental brain research     Volume:  154     ISSN:  0165-3806     ISO Abbreviation:  Brain Res. Dev. Brain Res.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-02-14     Completed Date:  2005-06-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8908639     Medline TA:  Brain Res Dev Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  247-54     Citation Subset:  IM    
Instituto de Biología Celular y Neurociencias Prof. E. De Robertis, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, (1121) Buenos Aires, Argentina.
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MeSH Terms
Animals, Newborn
Brain / drug effects*,  metabolism
Carbachol / pharmacology*
Cholinergic Agonists / pharmacology*
Dose-Response Relationship, Drug
Drug Interactions
Hydrolysis / drug effects
Inositol Phosphates / pharmacokinetics
Neurotensin / pharmacology
Phosphatidylinositols / metabolism*
Pyrazoles / pharmacology
Quinolines / pharmacology
Rats, Wistar
Receptors, Neurotensin / antagonists & inhibitors,  physiology*
Time Factors
Reg. No./Substance:
0/Cholinergic Agonists; 0/Inositol Phosphates; 0/Phosphatidylinositols; 0/Pyrazoles; 0/Quinolines; 0/Receptors, Neurotensin; 10028-17-8/Tritium; 146362-70-1/SR 48692; 39379-15-2/Neurotensin; 51-83-2/Carbachol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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