Document Detail


High affinity monoclonal antibodies to luteinizing hormone-releasing hormone. Preparation and binding studies.
MedLine Citation:
PMID:  6378971     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two mouse hybridoma cell lines (50-1 and 50-11) secreting high affinity, monoclonal IgG antibodies (MCAs) against luteinizing hormone-releasing hormone (LHRH) have been established. Measurements of binding affinity by both equilibrium and kinetic methods give dissociation constants (Kd) of 2.3-3.2 X 10(-10) M for 50-1 and 3.0 X 10(-10) M for 50-11. Analysis of binding specificity show that both MCAs require the C-terminal glycine amide and adjacent 4 amino acids of LHRH for binding. The use of these MCAs in radioimmunoassay (RIA) is described.
Authors:
R J Knapp; L A Sternberger
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neuroimmunology     Volume:  6     ISSN:  0165-5728     ISO Abbreviation:  J. Neuroimmunol.     Publication Date:  1984 Aug 
Date Detail:
Created Date:  1984-09-13     Completed Date:  1984-09-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8109498     Medline TA:  J Neuroimmunol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  361-71     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / immunology*
Cell Line
Gonadotropin-Releasing Hormone / analysis,  immunology*
Hypothalamus / analysis
Kinetics
Mice
Grant Support
ID/Acronym/Agency:
HD-12932/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 33515-09-2/Gonadotropin-Releasing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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