| High-affinity cytochalasin B binding to normal and transformed BALB/3T3 cells. | |
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MedLine Citation:
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PMID: 188845 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To study the molecular basis of changes in sugar uptake rate in cultured mouse fibroblasts with different physiological states, we have measured the high affinity binding of [3H] cytochalsin B, a potent sugar transport inhibitor, to actively growing and contact inhibited Balb/3T3 cells as well as to 3T12 and SV3T3 cells. Binding was the same whether the cells were detached from dishes with EDTA or trypsin. The amount of drug bound to intact cells measured with a centrifugation assay was essentially the same as that bound to cell sonicates measured with equilibrium dialysis. Cytochalasin B binding to intact cells was extremely rapid and reversible over a wide range of drug concentrations, and was not affected by 0.1 M D--glucose in the assay medium. Actively growing and contact inhibited 3T3 cells had a similar number of high affinity cytochalasin B binding sites per cell, while 3T12 and SV3T3 cells had one third to one fourth the number of sites per cell. However, the number of sites per mug cellular protein appeared to be similar for cells in all of the physiological states examined. |
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Authors:
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S J Atlas; S Lin |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of cellular physiology Volume: 89 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1976 Dec |
Date Detail:
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Created Date: 1977-03-21 Completed Date: 1977-03-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 751-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Binding Sites Cell Line Cell Transformation, Neoplastic* Contact Inhibition Cytochalasin B / metabolism* Fibroblasts / metabolism* Glucose / metabolism Mice Mice, Inbred BALB C Simian virus 40 Stereoisomerism |
| Chemical | |
Reg. No./Substance:
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14930-96-2/Cytochalasin B; 50-99-7/Glucose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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