Document Detail

High accumulation of plasminogen and tissue plasminogen activator at the flow surface of mural fibrin in the human arterial system.
MedLine Citation:
PMID:  10917998     Owner:  NLM     Status:  MEDLINE    
PURPOSE: We assessed the fibrinolytic activity of the organized mural thrombus lining of aneurysms and prosthetic grafts.
METHODS: Between May 1995 and April 1998, the full-thickness mural thrombi of aneurysms and the pseudointima lining of vascular grafts were obtained from 12 patients, ranging from 55 to 78 years in age, who underwent elective surgery. These included five aortic arch aneurysms, four abdominal aortic aneurysms, and three patent synthetic vascular grafts. The specimens were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)/immunoblot and immunohistochemistry for human plasmin/plasminogen, tissue plasminogen activator (tPA), and fibrin degradation product (D-dimer).
RESULTS: In the SDS-PAGE/immunoblot, 25- and 27-kd bands appeared specifically in experimental fibrin plates after limited digestion by plasmin and were also recognized in the mural thrombi. The presence of bands at 25 and 27 kd, which were most prominent in sections near the flow surface layer, was consistent with the hypothesis that the mural fibrin was digested by the endogenous plasmin. Apparent immunoreactivity was found at the flow surface of the masses at a thickness of 10 to 400 micrometer suggesting the presence of a plasminogen and tPA-rich layer, with D-dimer as a consequential product of fibrinolysis.
CONCLUSION: The hypothesis that fibrin surfaces in the arterial system acquire fibrinolytic activity because of digestion by circulating endogenous plasmin was confirmed; this may contribute to the antithrombogenicity of these flow surfaces.
T Sasajima; Y Takano; Y Hiraishi; K Goh; M Inaba; N Azuma; Y Sasajima; K Yamazaki; H Yamamoto
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of vascular surgery     Volume:  32     ISSN:  0741-5214     ISO Abbreviation:  J. Vasc. Surg.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-09-19     Completed Date:  2000-09-19     Revised Date:  2012-10-03    
Medline Journal Info:
Nlm Unique ID:  8407742     Medline TA:  J Vasc Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  374-82     Citation Subset:  IM    
First Department of Surgery, Asahikawa Medical College, and the R and D Center, the Terumo Corporation, Asahikawa, Japan.
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MeSH Terms
Aneurysm / metabolism*,  pathology*
Arteries / metabolism*
Blood Vessel Prosthesis*
Fibrin / metabolism*
Fibrinolysin / physiology
Fibrinolysis / physiology*
Middle Aged
Pancreatic Elastase / metabolism
Plasminogen / metabolism*
Regional Blood Flow
Thrombosis / metabolism*,  pathology*
Tissue Plasminogen Activator / metabolism*
Reg. No./Substance:
9001-31-4/Fibrin; 9001-91-6/Plasminogen; EC Elastase; EC Plasminogen Activator; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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