| High-Throughput Screening Assay for Sphingosine Kinase Inhibitors in Whole Blood Using RapidFire(R) Mass Spectrometry. | |
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MedLine Citation:
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PMID: 21297110 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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To facilitate discovery of compounds modulating sphingosine-1-phosphate (S1P) signaling, the authors used high-throughput mass spectrometry technology to measure S1P formation in human whole blood. Since blood contains endogenous sphingosine (SPH) and S1P, mass spectrometry was chosen to detect the conversion of an exogenously added 17-carbon-long variant of sphingosine, C17SPH, into C17S1P. The authors developed procedures to achieve homogeneous mixing of whole blood in 384-well plates and for a method requiring minimal manipulations to extract S1P from blood in 96- and 384-well plates prior to analyses using the RapidFire(®) mass spectrometry system. |
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Authors:
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Maureen K Highkin; Matthew P Yates; Olga V Nemirovskiy; William A Lamarr; Grace E Munie; John W Rains; Jaime L Masferrer; Marek M Nagiec |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biomolecular screening : the official journal of the Society for Biomolecular Screening Volume: 16 ISSN: 1552-454X ISO Abbreviation: J Biomol Screen Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-02-07 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9612112 Medline TA: J Biomol Screen Country: United States |
Other Details:
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Languages: eng Pagination: 272-7 Citation Subset: IM |
Affiliation:
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1Pfizer Global R&D, St. Louis, MO, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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