Document Detail

High-throughput RNA in situ hybridization in mouse retina.
MedLine Citation:
PMID:  23150371     Owner:  NLM     Status:  MEDLINE    
The introduction of large-scale gene expression profiling studies has greatly increased the need to rapidly obtain high-quality cellular expression patterns of genes found to exhibit differential expression. The use of large-scale nonradioactive RNA in situ hybridization makes this possible, and greatly increases the general usefulness of this data. Here, we describe protocols for parallel analysis of up to 50 different gene-specific probes in mouse retinal sections.
Seth Blackshaw
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  935     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-15     Completed Date:  2013-04-17     Revised Date:  2014-01-31    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  215-26     Citation Subset:  IM    
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MeSH Terms
Frozen Sections / methods
High-Throughput Screening Assays / methods*
In Situ Hybridization / methods*
Polymerase Chain Reaction / methods
RNA / analysis*,  genetics
Retina / metabolism*
Tissue Fixation / methods
Grant Support
R01 EY017015/EY/NEI NIH HHS; R01 EY020560/EY/NEI NIH HHS
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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