| High-resolution spatial measurements of ventilation-perfusion heterogeneity in rats. | |
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MedLine Citation:
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PMID: 20203067 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study was designed to validate a high-resolution method to measure regional ventilation (VA) in small laboratory animals, and to compare regional Va and perfusion (Q) before and after methacholine-induced bronchoconstriction. A mixture of two different colors of 0.04-microm fluorescent microspheres (FMS) was aerosolized and administered to five anesthetized, mechanically ventilated rats. Those rats also received an intravenous injection of a mixture of two different colors of 15-microm FMS to measure regional blood flow (Q). Five additional rats were labeled with aerosol and intravenous FMS, injected with intravenous methacholine, and then relabeled with a second pair of aerosol and intravenous FMS colors. After death, the lungs were reinflated, frozen, and sequentially sliced in 16-microm intervals on an imaging cryomicrotome set to acquire signal for each of the FMS colors. The reconstructed lung images were sampled using randomly placed 3-mm radius spheres. Va within each sphere was estimated from the aerosol fluorescence signal, and Q was estimated from the number of 15-microm FMS within each sphere. Method error ranged from 6 to 8% for Q and 0.5 to 4.0% for Va. The mean coefficient of variation for Q was 17%, and for Va was 34%. The administration of methacholine altered the distribution of both VA and Q within lung regions, with a change in Va distribution nearly twice as large as that seen for Q. The methacholine-induced changes in Va were not associated with compensatory shifts in Q. Cryomicrotome images of FMS markers provide a high-resolution, anatomically specific means of measuring regional VA/Q responses in the rat. |
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Authors:
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H Thomas Robertson; Melissa A Krueger; Wayne J E Lamm; Robb W Glenny |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Validation Studies Date: 2010-03-04 |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: 108 ISSN: 1522-1601 ISO Abbreviation: J. Appl. Physiol. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-05 Completed Date: 2010-08-12 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 1395-401 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of Washington, and University Hospital, Box 356522, Seattle, WA 98195-6522, USA. tomrobt@u.washington.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Inhalation Aerosols Animals Bronchoconstriction Bronchoconstrictor Agents / administration & dosage Fluorescent Dyes / administration & dosage Frozen Sections Image Processing, Computer-Assisted Injections, Intravenous Lung / blood supply*, drug effects, physiology* Male Methacholine Chloride / administration & dosage Microspheres Pulmonary Circulation* Rats Rats, Sprague-Dawley Regional Blood Flow Reproducibility of Results Respiration, Artificial Ventilation-Perfusion Ratio* |
| Grant Support | |
ID/Acronym/Agency:
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HL073598/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Aerosols; 0/Bronchoconstrictor Agents; 0/Fluorescent Dyes; 62-51-1/Methacholine Chloride |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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