Document Detail


High-resolution optical mapping of inflammatory macrophages following endovascular arterial injury.
MedLine Citation:
PMID:  23090852     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Inflammation following arterial injury mediates vascular restenosis, a leading cause of cardiovascular morbidity. Here we utilize intravital microscopy (IVM) and a dextran-coated nanosensor to spatially map inflammatory macrophages in vivo following endovascular injury of murine carotid arteries.
PROCEDURES: C57Bl/6 mice (n = 23) underwent endovascular guidewire carotid arterial injury. At day 14 or day 28 post-injury, mice underwent fluorescence IVM, 24 h after injection with the near-infrared fluorescent macrophage nanosensor CLIO-VT680. Adventitial collagen was concomitantly imaged using second harmonic generation (SHG) IVM. Correlative fluorescence microscopy and immunohistochemistry were performed.
RESULTS: Two-plane IVM reconstructions detected macrophage inflammation in the arterial wall that was elevated at day 14 compared to day 28 animals (P < 0.05). SHG-based collagen imaging of the outer arterial wall facilitated analysis of the macrophage-rich, inflamed neointima. Histological analyses and fluorescence microscopy data demonstrated increased macrophage infiltration in day 14 compared to day 28 neointima.
CONCLUSIONS: We demonstrate that the macrophage response to arterial injury can be imaged in vivo using IVM-based molecular imaging, and shows a higher macrophage influx at day 14 compared to day 28 post-injury.
Authors:
Amit Saxena; Chase W Kessinger; Brian Thompson; Jason R McCarthy; Yoshiko Iwamoto; Charles P Lin; Farouc A Jaffer
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging     Volume:  15     ISSN:  1860-2002     ISO Abbreviation:  Mol Imaging Biol     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-05-08     Completed Date:  2014-02-20     Revised Date:  2014-06-03    
Medline Journal Info:
Nlm Unique ID:  101125610     Medline TA:  Mol Imaging Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  282-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carotid Arteries / pathology*
Carotid Artery Injuries / diagnosis*
Cell Proliferation
Endothelial Cells / pathology
Endothelium, Vascular / pathology
Image Processing, Computer-Assisted
Inflammation / pathology*
Macrophages / pathology*
Mice
Mice, Inbred C57BL
Microscopy, Fluorescence
Neointima / pathology
Optical Imaging / methods*
Vascular System Injuries / diagnosis*,  pathology
Grant Support
ID/Acronym/Agency:
P41 EB015903/EB/NIBIB NIH HHS; R01 HL-108229/HL/NHLBI NIH HHS; R01 HL108229/HL/NHLBI NIH HHS; T32 HL-076136/HL/NHLBI NIH HHS; //Howard Hughes Medical Institute
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