| High Resolution Magnetic Resonance Imaging Quantitatively Detects Individual Pancreatic Islets. | |
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MedLine Citation:
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PMID: 21926272 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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OBJECTIVEWe studied whether manganese-enhanced high field magnetic resonance (MR) imaging (MEHFMRI) could quantitatively detect individual islets in situ and in vivo and evaluate changes in a model of experimental diabetes.RESEARCH DESIGN AND METHODSWhole pancreas from untreated (n = 3), MnCl(2) and glucose-injected mice (n = 6), and mice injected with either streptozotocin (STZ; n = 4) or citrate buffer (n = 4) were imaged ex vivo for unambiguous evaluation of islets. Exteriorized pancreas of MnCl(2) and glucose-injected mice (n = 6) were imaged in vivo to directly visualize the gland and minimize movements. In all cases, MR images were acquired in a 14.1 Tesla scanner and correlated with the corresponding (immuno)histological sections.RESULTSIn ex vivo experiments, MEHFMRI distinguished different pancreatic tissues and evaluated the relative abundance of islets in the pancreas of normoglycemic mice. MEHFMRI also detected a significant decrease in the numerical and volume density of islets in STZ-injected mice. However, in the latter measurements the loss of β-cells was undervalued under the conditions tested. The experiments on the externalized pancreas confirmed that MEHFMRI could visualize native individual islets in living, anesthetized mice.CONCLUSIONSData show that MEHFMRI quantitatively visualizes individual islets in the intact mouse pancreas, both ex vivo and in vivo. |
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Authors:
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Smaragda Lamprianou; Riikka Immonen; Christine Nabuurs; Asllan Gjinovci; Laurent Vinet; Xavier C R Montet; Rolf Gruetter; Paolo Meda |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-16 |
Journal Detail:
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Title: Diabetes Volume: - ISSN: 1939-327X ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372763 Medline TA: Diabetes Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Cell Physiology and Metabolism, University of Geneva, Geneva, Switzerland. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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