Document Detail


High-performance liquid chromatography-tandem mass spectrometry method for simultaneous quantification of carbamazepine, oxcarbazepine, and their main metabolites in human serum.
MedLine Citation:
PMID:  22210100     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Antiepileptic drug therapeutic regimens often need to be adjusted individually on the basis of serum assays. We aimed to develop a quantitative, fast, and sensitive liquid chromatography-tandem mass spectrometry method to simultaneously analyze carbamazepine, oxcarbazepine, and the 10-11 epoxide carbamazepine and 10-hydroxy carbazepine (mono-hydroxy derivative, 10,11-Dihydro-10-hydroxycarbamazepine) metabolites, in human serum.
METHODS: Serum samples were deproteinized by acetonitrile spiked with dansyl-norvaline as internal standard. Compounds were separated on a reversed-phase high-performance liquid chromatography over a total run time of 10 minutes. Serum concentrations were then measured by means of a triple quadrupole tandem mass spectrometer, set up in positive mode and multiple reaction monitoring.
RESULTS: Calibration curves (0.08-50 mcg/mL for carbamazepine and 10,11-dihydro-10-hydroxycarbamazepine; 0.03-20 mcg/mL for oxcarbazepine and epoxide carbamazepine) were linear, with a mean correlation coefficient >0.999. Both the intra- and interassay imprecision and inaccuracy were within 10%. The absolute recovery ranged from 98% to 103% for all analytes.
CONCLUSIONS: The method requires minimal sample preparation. Volume of the sample is lower and run time shorter than required by previous published liquid chromatography-tandem mass spectrometry methods. Results are accurate. The method seems, therefore, to be reliable and economically suitable for routine analysis of antiepileptic drugs monitoring in clinical settings.
Authors:
Luana Lionetto; Barbara Casolla; Michele Cavallari; Paolo Tisei; Carla Buttinelli; Maurizio Simmaco
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Therapeutic drug monitoring     Volume:  34     ISSN:  1536-3694     ISO Abbreviation:  Ther Drug Monit     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-17     Completed Date:  2012-05-15     Revised Date:  2013-08-21    
Medline Journal Info:
Nlm Unique ID:  7909660     Medline TA:  Ther Drug Monit     Country:  United States    
Other Details:
Languages:  eng     Pagination:  53-8     Citation Subset:  IM    
Affiliation:
Department of Neurosciences, Mental Health and Sensory Organs and UOD Diagnostica Molecolare Avanzata-Azienda Ospedaliera Sant'Andrea, University Sapienza of Rome, Rome, Italy.
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MeSH Terms
Descriptor/Qualifier:
Anticonvulsants / blood,  metabolism
Carbamazepine / analogs & derivatives*,  blood*,  metabolism*
Chromatography, High Pressure Liquid / methods*
Humans
Sensitivity and Specificity
Tandem Mass Spectrometry / methods*
Chemical
Reg. No./Substance:
0/Anticonvulsants; 298-46-4/Carbamazepine; VZI5B1W380/oxcarbazepine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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