Document Detail

A high omega-3 fatty acid diet has different effects on early and late stage myeloid progenitors.
MedLine Citation:
PMID:  21038084     Owner:  NLM     Status:  MEDLINE    
The effects of the polyunsaturated omega-3 (n-3) and omega-6 (n-6) fatty acids (FA) on hematopoiesis are complex in that both FA forms are processed into leukotrienes, eicosanoids, and prostaglandins, which can have independent effects. These FA have antagonistic effects in that n-6 FA prostaglandins tend to be pro-proliferative and pro-inflammatory, while the effects of n-3 FA prostaglandins are the opposite. We have previously shown that diets high in n-3 FA reduce the size of the middle to later stage myeloid progenitor compartment in FVB X sv129 F(1)hybrid mice. To assay the effects of high n-3 FA diets on earlier stages of myelopoiesis, we fed C57BL/6J mice diets high in n-3 FA or levels of n-3/n-6 FA similar to western diets and assayed the effects on myelopoiesis with flow cytometry and colony forming cell assays. Results indicate an expansion of the common myeloid progenitor cell compartment in high n-3 FA diets, which does not persist into later stages where the number of progenitor cells is actually lower in high n-3 FA fed animals. Investigations in vitro with the hematopoietic stem cell line EML-clone 1 indicate that cells cultured with eicosapentaenoic acid (n-3 FA) or arachidonic acid (n-6 FA) have no differences in cell viability but that arachidonic acid more rapidly produces progenitors with low levels of the macrophage developmental marker, F4/80.
Melinda E Varney; James T Buchanan; Yulia Dementieva; W Elaine Hardman; Vincent E Sollars
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-10-31
Journal Detail:
Title:  Lipids     Volume:  46     ISSN:  1558-9307     ISO Abbreviation:  Lipids     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-21     Completed Date:  2011-06-28     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  47-57     Citation Subset:  IM    
Department of Biochemistry and Microbiology, Marshall University School of Medicine, Huntington, WV 25755, USA.
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MeSH Terms
Antigens, Differentiation / metabolism
Arachidonic Acid / metabolism
Cell Differentiation
Eicosapentaenoic Acid / metabolism
Fatty Acids, Omega-3 / administration & dosage,  pharmacology*
Fatty Acids, Omega-6 / metabolism
Flow Cytometry
Mice, Inbred C57BL
Myeloid Progenitor Cells / drug effects*,  metabolism
Myelopoiesis / physiology
Grant Support
1R01 CA114018/CA/NCI NIH HHS; 1R03 CA129790/CA/NCI NIH HHS; 5P20RR016477/RR/NCRR NIH HHS; 5P20RR020180/RR/NCRR NIH HHS; P20 RR016477-10/RR/NCRR NIH HHS; P20 RR020180-05S1/RR/NCRR NIH HHS; R01 CA114018-04/CA/NCI NIH HHS; R03 CA129790-02/CA/NCI NIH HHS
Reg. No./Substance:
0/Antigens, Differentiation; 0/Fatty Acids, Omega-3; 0/Fatty Acids, Omega-6; 0/monocyte-macrophage differentiation antigen; 1553-41-9/Eicosapentaenoic Acid; 506-32-1/Arachidonic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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