| A high omega-3 fatty acid diet has different effects on early and late stage myeloid progenitors. | |
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MedLine Citation:
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PMID: 21038084 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of the polyunsaturated omega-3 (n-3) and omega-6 (n-6) fatty acids (FA) on hematopoiesis are complex in that both FA forms are processed into leukotrienes, eicosanoids, and prostaglandins, which can have independent effects. These FA have antagonistic effects in that n-6 FA prostaglandins tend to be pro-proliferative and pro-inflammatory, while the effects of n-3 FA prostaglandins are the opposite. We have previously shown that diets high in n-3 FA reduce the size of the middle to later stage myeloid progenitor compartment in FVB X sv129 F(1)hybrid mice. To assay the effects of high n-3 FA diets on earlier stages of myelopoiesis, we fed C57BL/6J mice diets high in n-3 FA or levels of n-3/n-6 FA similar to western diets and assayed the effects on myelopoiesis with flow cytometry and colony forming cell assays. Results indicate an expansion of the common myeloid progenitor cell compartment in high n-3 FA diets, which does not persist into later stages where the number of progenitor cells is actually lower in high n-3 FA fed animals. Investigations in vitro with the hematopoietic stem cell line EML-clone 1 indicate that cells cultured with eicosapentaenoic acid (n-3 FA) or arachidonic acid (n-6 FA) have no differences in cell viability but that arachidonic acid more rapidly produces progenitors with low levels of the macrophage developmental marker, F4/80. |
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Authors:
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Melinda E Varney; James T Buchanan; Yulia Dementieva; W Elaine Hardman; Vincent E Sollars |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-10-31 |
Journal Detail:
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Title: Lipids Volume: 46 ISSN: 1558-9307 ISO Abbreviation: Lipids Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-21 Completed Date: 2011-06-28 Revised Date: 2012-01-04 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: Germany |
Other Details:
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Languages: eng Pagination: 47-57 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Microbiology, Marshall University School of Medicine, Huntington, WV 25755, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Differentiation / metabolism Arachidonic Acid / metabolism Cell Differentiation Diet Eicosapentaenoic Acid / metabolism Fatty Acids, Omega-3 / administration & dosage, pharmacology* Fatty Acids, Omega-6 / metabolism Flow Cytometry Mice Mice, Inbred C57BL Myeloid Progenitor Cells / drug effects*, metabolism Myelopoiesis / physiology Phenotype |
| Grant Support | |
ID/Acronym/Agency:
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1R01 CA114018/CA/NCI NIH HHS; 1R03 CA129790/CA/NCI NIH HHS; 5P20RR016477/RR/NCRR NIH HHS; 5P20RR020180/RR/NCRR NIH HHS; P20 RR016477-10/RR/NCRR NIH HHS; P20 RR020180-05S1/RR/NCRR NIH HHS; R01 CA114018-04/CA/NCI NIH HHS; R03 CA129790-02/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Differentiation; 0/Fatty Acids, Omega-3; 0/Fatty Acids, Omega-6; 0/monocyte-macrophage differentiation antigen; 1553-41-9/Eicosapentaenoic Acid; 506-32-1/Arachidonic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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