Document Detail


High Level of Heterogeneity Among Listeria monocytogenes Isolates from Clinical and Food Origin Specimens in Greece.
MedLine Citation:
PMID:  22880958     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract In order to examine the genetic variation of clinical and food isolates of Listeria monocytogenes in Greece, a total of 61 L. monocytogenes non-duplicate isolates, recovered from clinical specimens (n=19) and food (n=42), were serotyped and genotyped using two different Random Amplification of Polymorphic DNA (RAPD) protocols and Multiple Locus Variable Number Tandem Repeat Analysis (MLVA). Serotype group 4b, 4d, 4e prevailed (39.4%), among both clinical and food isolates, followed by serotype group 1/2a, 3a (23.0%), which nevertheless was detected only among food isolates. The most discriminatory typing protocol was MLVA, which grouped four isolates into two pairs, while the remaining isolates produced unique fingerprints. Similar results were obtained when taking into account the combination of the two RAPD protocols (Simpson index 0.999); six isolates were grouped into three pairs, two of which were the pairs that were identified also by MLVA. Single use of each RAPD protocol resulted in inferior discrimination (Simpson index 0.978 and 0.997, respectively). In conclusion, the two molecular procedures, MLVA, and the combined RAPD protocols, produced similar results, showing that L. monocytogenes isolates from clinical and food specimens were highly heterogenous and that clustering was very uncommon.
Authors:
Dimitra P Houhoula; Dimitra Peirasmaki; Spyros J Konteles; Dimosthenis Kizis; Stamatis Koussissis; Michael Bratacos; Nikolaos Poggas; Ekaterina Charvalos; Athanassios Tsakris; Joseph Papaparaskevas
Related Documents :
18602678 - Rate-dependent effects of bupropion on nicotine self-administration and food-maintained...
16811988 - Pigeons' preferences for stimulus information: effects of amount of information.
23107718 - Physiochemical properties and prolonged release behaviours of chitosan-denatured β-lac...
7714448 - Conditioned reinforcement and choice with delayed and uncertain primary reinforcers.
7193858 - The effect of ambient temperature on rectal temperature, food intake and short term bod...
2967918 - A case report on a minor contamination of nivalenol in cereals harvested in canada.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-10
Journal Detail:
Title:  Foodborne pathogens and disease     Volume:  -     ISSN:  1556-7125     ISO Abbreviation:  Foodborne Pathog. Dis.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101120121     Medline TA:  Foodborne Pathog Dis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1 Department of Food Technology, Technological Educational Institute of Athens , Aigaleo, Greece .
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Use of Care Management Practices in Small- and Medium-Sized Physician Groups: Do Public Reporting of...
Next Document:  Robust Surface Nano-Architecture by Alkali-Carboxylate Ionic Bonding.